Submitted on October 10, 2007
Accepted on February 2, 2008
DYNAMICS OF ENTEROCYTE TIGHT JUNCTIONS - EFFECT OF EXPERIMENTAL COLITIS AND TWO DIFFERENT ANTI-TNF STRATEGIES
Walter Fries1*, Carmelo Muja2, Carmela Crisafulli3, Salvatore Cuzzocrea3, and Emanuela Mazzon3
1 Dip di Medicina Interna e Terapia Medica, University of Messina, Messina, Italy
2 Dip Clinico-Sperimentale di Medicina e Farmacologia, Sez di Farmcologia, University of Messina, Messina, Italy
3 Messina, Italy; Dip Clinico-Sperimentale di Medicina e Farmacologia, Sez di Farmcologia, University of Messina, Messina, Italy
* To whom correspondence should be addressed. E-mail: fwalter{at}unime.it.
An alteration of the intestinal barrier is considered to represent an early step in pathogenesis of Crohn's disease. The integrity of intestinal barrier function is guaranteed among other factors by enterocyte tight junction (TJ) proteins. Clinical and experimental data indicate the tumor necrosis factor-
(TNF-) to be the major responsible for these defects. In the present study we investigated the very early effects of DNBS/ethanol colitis on ileal enterocyte TJ proteins (occludin, ZO-1 claudin-2) in controls, mice treated with infliximab (IFX) or with etanercept (ETC), and in mice k.o. for the receptor 1 (TNFR-1-/- ). Circulating TNF- levels were effectively reduced by IFX and ETC (p<0.01, both) at 3 and at 6 h. DNBS colitis induced disappearance of occludin and ZO-1 from enterocyte cell-cell contact, whereas claudin-2, absent under control conditions, appeared in the ileal epithelium. These alterations were prevented equally by both treatments, IFX and ETC, and in TNFR-1-/- animals. DNBS colitis induced a very rapid loss of occludin and ZO-1 from ileal TJ together with an upregulation of claudin-2. Our data are consistent with the hypothesis that TNF- is involved in early TJ rearrangement and that its effects are mediated through TNFR-1. Despite clinical differences, both anti-TNF treatments were equally effective in the present setting.
