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Am J Physiol Gastrointest Liver Physiol 234: G365-G369, 1978;
0193-1857/78 $5.00
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AJP: Gastrointestinal and Liver Physiology, Vol 234, Issue 4, G365-G369
Copyright © 1978 by American Physiological Society

ARTICLES

A potential artifact in use of a new technique for rapid brain removal

KL Wong and GM Tyce

New techniques are are continuously being developed to obtain animal brain tissue that is as close as possible to its in vivo status. The brain-blowing technique provides a swift freezing of brain tissue (within 1 s) in which postmortem changes have been said to be minimal. Higher concentrations of glucose were present in brain samples removed by this method than have previously been found when the brain was frozen in situ, but at the same time the plasma levels of glucose were higher. It is suggested that, although the procedure did not appear to involve any stress, the hyperglycemia was stress related. Modifications of the procedure are described, by which these changes in blood glucose have been minimized.




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