AJP - Gastrointestinal and Liver Physiology, Vol 240, Issue 2 114-G121, Copyright © 1981 by American Physiological Society
Hormone-stimulated release of pancreatic polypeptide before and after vagotomy in dogs
S. Guzman, J. Lonovics, P. G. Devitt, K. E. Hejtmancik, P. L. Rayford and J. C. Thompson
Concentrations of pancreatic polypeptide (PP) in peripheral blood were
measured before, during, and after infusions of graded doses of synthetic
human gastrin I (SHG-I), cholecystokinin 99% pure (CCK-99%), CCK
octapeptide (CCK-OP), and pure natural porcine secretin in six dogs with
gastric and duodenal fistulas. Studies were repeated after truncal
vagotomy. Significant increases in concentrations of PP were found with 1
microgram . kg-1 . h-1 of SHG-I, 0.25 and 1.0 microgram . kg-1 . h-1 of
CCK-99%, and 0.06 and 0.25 micrograms . kg-1 . h-1 of CCK-OP. Significant
increases persisted after vagotomy, except at the lower dose of CCK-OP.
Postvagotomy responses were significantly less than prevagotomy, except at
the higher doses of CCK-99% and CCK-OP. Pure secretin did not change
concentrations of PP in blood before or after vagotomy. The most potent
stimulant for PP release on a molar basis was CCI-99%, followed by CCK-OP
and SHG-I. The results suggest that cholinergic and humoral agents of the
gastrin-cholecystokinin family interact in the normal physiological
response of PP to food and that, in dogs, CCK-like peptides are more potent
than gastrin.
