AJP - Gastrointestinal and Liver Physiology, Vol 240, Issue 6 466-G471, Copyright © 1981 by American Physiological Society
Reversal of cholecystokinin-induced persistent stimulation of pancreatic enzyme secretion by dibutyryl cyclic GMP
S. M. Collins, S. Abdelmoumene, R. T. Jensen and J. D. Gardner
When pancreatic acini are first incubated with cholecystokinin, washed to
remove free cholecystokinin, and then reincubated in fresh incubation
solution, there is a significant residual stimulation of amylase secretion.
Butyryl derivatives of cyclic GMP can prevent as well as reverse this
cholecystokinin-induced residual stimulation. At 37 degrees C the
nucleotide-induced reversal is complete within a few minutes, but at 4
degrees C complete reversal requires 90 min of incubation. The ability of
butyryl cyclic GMP to reverse cholecystokinin-induced residual stimulation
is itself fully reversible, and the nucleotide-induced reversal is
accompanied by restoration of full responsiveness to cholecystokinin. The
ability of dibutyryl cyclic GMP to reverse cholecystokinin-induced residual
stimulation appears to result from the ability of the nucleotide to
displace cholecystokinin from its receptors in pancreatic acini.
