AJP - GI AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 242: G47-G51, 1982;
0193-1857/82 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sinar, D. R.
Right arrow Articles by Burns, T. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sinar, D. R.
Right arrow Articles by Burns, T. W.

AJP - Gastrointestinal and Liver Physiology, Vol 242, Issue 1 47-G51, Copyright © 1982 by American Physiological Society

ARTICLES

Migrating action-potential complex activity in absence of fluid production is produced by B subunit of cholera enterotoxin

D. R. Sinar, L. G. Charles and T. W. Burns

The myoelectric response and fluid output from in vivo rabbit ileal loops injected with B subunits of purified cholera enterotoxin are compared with the response to the purified cholera holotoxin. Migrating action-potential complex (MAPC) frequency is similar after injection of purified cholera toxin or B subunits. In contrast, fluid output after B-subunit injection is significantly (P less than 0.05) less than fluid output after purified cholera toxin injection. Specific antiserum to the holotoxin incubated with holotoxin at equivalence significantly decreased both MAPC activity (P less than 0.05) and fluid output (P less than 0.001) from the purified toxin. Purified cholera toxin and B subunits, modified by reaction with 2-nitrophenylsulfonyl chloride to produce monomeric B fractions with decreased GM1 ganglioside-binding properties, produced significantly (P less than 0.05) less fluid output and MAPC activity. Binding of the toxin or B subunits in the aggregated form is essential for the expression of MAPC activity or fluid output. These results suggest that the B subunit of cholera toxin produces MAPC activity in the rabbit ileum in the absence of fluid production. Furthermore, previous assumptions that MAPC activity is linked to fluid secretion should be reconsidered. It appears that the gut responses to cholera toxin, fluid production, or MAPC activity are produced by separate mechanisms.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. H. Percy, R. Burakoff, K. Rose, H. P. Desai, C. Pothoulakis, and R. Eglow
In vitro evidence that rabbit distal colonic muscularis mucosae has a Clostridium difficile toxin A receptor
Am J Physiol Gastrointest Liver Physiol, September 1, 1998; 275(3): G402 - G409.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online