AJP - Gastrointestinal and Liver Physiology, Vol 242, Issue 4 313-G318, Copyright © 1982 by American Physiological Society

ARTICLES

Hemodynamic effects on oxygen consumption and bile flow in isolated skate liver

J. S. Reed, N. D. Smith and J. L. Boyer

A technique is described for perfusion of livers from the little skate, Raja erinacea, in the isolated state at 15 degrees C utilizing a recirculating, well-oxygenated elasmobranch Ringer solution. Bile flow and oxygen consumption were optimal and remained relatively constant over a 4- to 5-h perfusion at portal vein pressures of 2.5-5.0 cm Ringer. At these pressures, bile flow rates were comparable to values previously observed in the free-swimming skate. In contrast to mammalian species, bile secretion in the elasmobranch is particularly sensitive to small changes in perfusion pressure. Hepatic clearance of sulfobromophthalein and [14C]sodium taurocholate demonstrated initial fractional disappearance rates that were also similar to values obtained in vivo. Only small amounts of sulfobromophthalein appeared in bile by 5 h, whereas 78.5 +/- 6.5% of [14C]sodium taurocholate was recovered in bile at this time. These experiments establish portal vein perfusion pressures for study of skate livers in the isolated state in which oxygen consumption is maximal and organic anion clearance from perfusate to bile exceeds values obtained in vivo.

This article has been cited by other articles:

				R. P. J. O. Elferink, R. Ottenhoff, G. Fricker, D. J. Seward, N. Ballatori, and J. Boyer Lack of biliary lipid excretion in the little skate, Raja erinacea, indicates the absence of functional Mdr2, Abcg5, and Abcg8 transporters Am J Physiol Gastrointest Liver Physiol, May 1, 2004; 286(5): G762 - G768. [Abstract] [Full Text] [PDF]

				S.-Y. Cai, C. J. Soroka, N. Ballatori, and J. L. Boyer Molecular characterization of a multidrug resistance-associated protein, Mrp2, from the little skate Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2003; 284(1): R125 - R130. [Abstract] [Full Text] [PDF]

				S.-Y. Cai, W. Wang, C. J. Soroka, N. Ballatori, and J. L. Boyer An evolutionarily ancient Oatp: insights into conserved functional domains of these proteins Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G702 - G710. [Abstract] [Full Text] [PDF]

				S.-Y. Cai, L. Wang, N. Ballatori, and J. L. Boyer Bile salt export pump is highly conserved during vertebrate evolution and its expression is inhibited by PFIC type II mutations Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G316 - G322. [Abstract] [Full Text] [PDF]

				N. Ballatori, D. N. Hager, S. Nundy, D. S. Miller, and J. L. Boyer Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited Am J Physiol Gastrointest Liver Physiol, October 1, 1999; 277(4): G896 - G904. [Abstract] [Full Text] [PDF]

				G. Fricker, R. Wossner, J. Drewe, R. Fricker, and J. L. Boyer Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea) Am J Physiol Gastrointest Liver Physiol, November 1, 1997; 273(5): G1023 - G1030. [Abstract] [Full Text] [PDF]