AJP - Gastrointestinal and Liver Physiology, Vol 244, Issue 4 392-G396, Copyright © 1983 by American Physiological Society
Pepsinogen secretion from dispersed glands from rabbit stomach
D. K. Kasbekar, R. T. Jensen and J. D. Gardner
In dispersed glands from rabbit stomach, pepsinogen secretion was
stimulated by carbamylcholine, the C-terminal octapeptide of
cholecystokinin (CCK-8), and structurally related peptides physalaemin and
A23187 but not by bombesin or histamine. Reducing the incubation
temperature from 37 degrees to 4 degrees C or adding carbonyl cyanide
m-chlorophenylhydrazone reduced basal as well as stimulated pepsinogen
secretion. Dibutyryl cGMP inhibited the stimulation of pepsinogen secretion
caused by cholecystokinin but not that caused by carbamylcholine; atropine
inhibited the stimulation of pepsinogen secretion caused by carbamylcholine
but not that caused by cholecystokinin. Each cholecystokinin-related
peptide had the same efficacy for stimulating pepsinogen secretion. In
terms of the concentration that caused half-maximal stimulation of
pepsinogen secretion, the relative potencies of cholecystokinin-related
peptides were caerulein greater than CCK-8 greater than [desSO3]CCK-8
greater than gastrin. Removing extracellular calcium did not alter basal or
cholecystokinin-stimulated pepsinogen secretion. These results illustrate
that dispersed glands from rabbit stomach constitute a suitable preparation
for examining the actions of various agents on pepsinogen secretion in
vitro.
