AJP - Gastrointestinal and Liver Physiology, Vol 245, Issue 4 463-G469, Copyright © 1983 by American Physiological Society
Antral gland cell column: a method for studying release of gastric hormones
B. Richelsen, J. F. Rehfeld and L. I. Larsson
A technique for studying in vitro release of gastric hormones has been
developed. The system utilizes nonenzymatically isolated antropyloric
glands from humans or rats, which are perifused in a Bio-Gel P-2 column.
The system permits the study of kinetics and dose-response characteristics
using the glands as their own control. The glands were stimulated with
carbachol and bombesin, and the antral peptides gastrin and somatostatin
were measured. Bombesin and carbachol both evoked a dose-dependent
stimulation of gastrin release, beginning at below 10(-10) M (bombesin) and
10(-7) M (carbachol). Carbachol inhibited the release of somatostatin in a
dose-dependent manner, being maximally effective at 10(-6) M and then
producing 60% inhibition of somatostatin release. Bombesin was without
effect on antropyloric somatostatin release. These data suggest that the
gastrin-stimulating effect of carbachol is partially or totally due to
inhibition of somatostatin release, whereas bombesinergic stimulation of
gastrin release must work in an independent manner. In addition, data on
the effects of these substances on the release of gastrin and ACTH-like
peptides from human antropyloric glands are presented. Due to the absence
of local neural reflexes, this system is a useful supplement to the
isolated perfused stomach model.
