AJP - Gastrointestinal and Liver Physiology, Vol 247, Issue 3 226-G230, Copyright © 1984 by American Physiological Society

ARTICLES

Effect of streptozotocin-induced diabetes on bile acid sulfation in male rat liver

R. B. Kirkpatrick and B. G. Kraft

The sulfation of bile acids is hormone dependent, being increased in females and ethynylestradiol (EE)-treated males compared with normal males. Diabetes causes significant alterations in estrogen metabolism and uterine estrogen receptor kinetics. Male rats were given streptozotocin (90 mg/kg) and diabetes was verified. An increase in hepatic bile acid sulfotransferase (BAST) activity was significant by 6 days and continued to increase to 29 days. This increase was prevented by insulin replacement. Administration of EE (6.0-600 micrograms X kg-1 X day-1) to normal male rats resulted in a significant increase in hepatic BAST activity; however, administration of similar doses of EE to diabetic males failed to further increase activity levels over the already-elevated levels in the diabetic controls. This increase in in vitro specific activity was accompanied by an increase in the biliary excretion of lithocholate 3-sulfate and taurolithocholate 3-sulfate in 21-day-diabetic animals. Bile flow and total bile acid excretion were also markedly increased in the diabetic animals. The data indicate that streptozotocin-induced diabetes causes a significant increase in hepatic BAST activity. These findings are consistent with an alteration in hepatic estrogen action in streptozotocin-induced diabetes.

This article has been cited by other articles:

				J. L. Stone, J. B. Braunstein, T. M. Beaty, R. A. Sanders, and J. B. Watkins III Hepatobiliary Excretion of Bile Acids and Rose Bengal in Streptozotocin-Induced and Genetic Diabetic Rats J. Pharmacol. Exp. Ther., April 1, 1997; 281(1): 412 - 419. [Abstract] [Full Text]