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Am J Physiol Gastrointest Liver Physiol 247: G394-G401, 1984;
0193-1857/84 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 247, Issue 4 394-G401, Copyright © 1984 by American Physiological Society

ARTICLES

Characteristics of cysteine uptake in intestinal basolateral membrane vesicles

L. H. Lash and D. P. Jones

Uptake of cysteine in intestinal basolateral membrane vesicles was independent of Na+, was sensitive to medium osmolarity, exhibited saturation kinetics, and was selectively inhibited by other amino acids in a concentration-dependent manner, indicating transport by a carrier-mediated process. The kinetics indicated the existence of two transport systems: a high- and a low-Km system with Km and Vmax values that differed by greater than one order of magnitude. The substrate specificity pattern indicated two principal transport systems for cysteine: one corresponding to the high-Km system and one to the low-Km system. The high-Km system was inhibited primarily by neutral amino acids with small or polar side chains (alanine, serine, threonine, and glycine), resembling the ASC system in its specificity. The low-Km system was inhibited primarily by neutral amino acids with large, nonpolar side chains (leucine, phenylalanine, and methionine) and by the leucine analogue beta-2-aminobicyclo(2,2,1)heptane-2-carboxylic acid, identifying it as the L system. The two systems also exhibited trans stimulation, but only with those amino acids that caused cis inhibition.


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