AJP - GI Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 247: G558-G566, 1984;
0193-1857/84 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soll, A. H.
Right arrow Articles by Thomas, L. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soll, A. H.
Right arrow Articles by Thomas, L. P.

AJP - Gastrointestinal and Liver Physiology, Vol 247, Issue 5 558-G566, Copyright © 1984 by American Physiological Society

ARTICLES

Release of somatostatinlike immunoreactivity from canine fundic mucosal cells in primary culture

A. H. Soll, T. Yamada, J. Park and L. P. Thomas

We have developed a model to allow study of the release of somatostatinlike immunoreactivity (SLI) from gastric mucosal cells. Collagenase-dispersed canine fundic mucosal cells were separated by counterflow elutriation. SLI-containing cells were identified in the fractions with small cells (9-11 microns), and these fractions were plated onto collagen. After 2 days in culture, SLI content of the cells was maintained; SLI-positive cells, detected by peroxidase-antiperoxidase immunohistochemistry, comprised 70 +/- 6% (mean +/- SE, n = 6) of these cultured cells. Release of SLI from these cultures into the medium was determined by radioimmunoassay. Epinephrine, dibutyryl cAMP, and gastrin each stimulated SLI release in a time-dependent manner, with a steady rate of secretion maintained for 120 min of incubation. Both epinephrine and dibutyryl cAMP markedly potentiated the release of SLI stimulated by gastrin but were not themselves mutually potentiating. Upon Sephadex G-50 column chromatography of incubation medium and extracts of cultured cells, SLI eluted primarily in a single peak that cochromatographed with synthetic somatostatin tetradecapeptide. Our data suggest that gastrin and adrenergic stimuli act directly on canine fundic somatostatin cells and that potentiating interactions between secretagogues may be important modulating elements in somatostatin cell secretory function.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Zavros, S. Rathinavelu, J. Y. Kao, A. Todisco, J. Del Valle, J. V. Weinstock, M. J. Low, and J. L. Merchant
Treatment of Helicobacter gastritis with IL-4 requires somatostatin
PNAS, October 28, 2003; 100(22): 12944 - 12949.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. L. Westbrook, G. H. McDowell, K. J. Hardy, and A. Shulkes
Active immunization against somatostatin alters regulation of gastrin in response to gastric acid secretagogues
Am J Physiol Gastrointest Liver Physiol, April 1, 1998; 274(4): G751 - G756.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online