AJP - Gastrointestinal and Liver Physiology, Vol 248, Issue 5 539-G544, Copyright © 1985 by American Physiological Society
Measurement of regional gastroduodenal blood flow with iodo [14C]antipyrine autoradiography
D. Hudson, O. U. Scremin and P. H. Guth
Regional blood flow was measured in the gastroduodenal area by means of
iodo[14C]antipyrine autoradiography. The tracer was given intravenously
over a short period of time in awake rats followed by cardiac arrest and
quick freezing of stomach and duodenum. Dry autoradiography of frozen
sections provide a pictorial representation of radioactivity in the
different tissue layers. This could be converted to blood flow after
incorporation of the time course of blood radioactivity into an operational
equation. A high level of resolution was achieved, allowing delineation of
blood flow compartments of small dimensions such as single villi and
regions within the fundic mucosa. The value of blood flow recorded in
resting conditions were in agreement with previous observations with the
microspheres technique. Hypotension induced a generalized decrease in blood
flow, most marked in the corpus mucosa.
