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Am J Physiol Gastrointest Liver Physiol 248: G551-G560, 1985;
0193-1857/85 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 248, Issue 5 551-G560, Copyright © 1985 by American Physiological Society

ARTICLES

Role of calcium in cholecystokinin-stimulated phosphoinositide breakdown in exocrine pancreas

S. J. Pandol, M. W. Thomas, M. S. Schoeffield, G. Sachs and S. Muallem

In dispersed acini from guinea pig pancreas cholecystokinin octapeptide (CCK-OP) stimulated breakdown of the phosphoinositides phosphatidylinositol (PI) and its phosphorylated derivative, phosphatidylinositol 4,5-bisphosphate (PI-P2), as measured by a decrease in the mass of PI and decreases in the content of [3H]PI and [32P]PI-P2 in acini prelabeled with myo-[2-3H]inositol or H3(32)PO4. The breakdown occurred in the absence of extracellular Ca2+ and when the CCK-OP-induced rise in free intracellular Ca2+ ([Ca2+]i) was ablated by loading the acini with the Ca2+-selective indicator and chelator quin-2 in the absence of extracellular Ca2+. In contrast to CCK-OP, the calcium ionophore A23187 caused breakdown of PI and PI-P2 in the presence but not in the absence of extracellular Ca2+, although like CCK-OP A23187 stimulated 45Ca outflux, a measure of cellular Ca2+ mobilization, and amylase release during the first 5-10 min of incubation independent of extracellular Ca2+. In the absence of extracellular Ca2+ A23187 did not inhibit the ability of CCK-OP to cause PI breakdown. These results indicate that CCK-OP stimulates breakdown of PI and PI-P2 and that this breakdown is independent of extracellular Ca2+, mobilization of intracellular Ca2+, and the CCK-OP-induced rise in [Ca2+]i. These findings suggest that one of the initial events resulting from CCK-OP interaction with its receptor is phosphoinositide breakdown.


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 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Satoh, A. S. Gukovskaya, J. M. Nieto, J. H. Cheng, I. Gukovsky, J. R. Reeve Jr, T. Shimosegawa, and S. J. Pandol
PKC-{delta} and -{epsilon} regulate NF-{kappa}B activation induced by cholecystokinin and TNF-{alpha} in pancreatic acinar cells
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G582 - G591.
[Abstract] [Full Text] [PDF]


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