AJP - Gastrointestinal and Liver Physiology, Vol 248, Issue 6 702-G708, Copyright © 1985 by American Physiological Society
Hepatic transport and binding of rose bengal in the presence of albumin and gamma globulin
E. L. Forker, B. A. Luxon and V. S. Sharma
Gamma globulin and albumin are compared with respect to their effects on
the hepatic transport of rose bengal and with respect to the rates and
affinities with which they bind this dye. The apparent intrinsic clearance
of rose bengal is greater in the presence of albumin than in the presence
of gamma globulin, and this difference increases with the protein
concentration. Because the binding affinities of these proteins also
differ, however, it cannot be concluded decisively that the mechanisms of
dye removal are distinct. For both proteins the binding reaction rates as
measured by stopped-flow spectrophotometry are much faster than the rate of
convection along the sinusoid or the rate of removal of free dye by liver
cells. The transport data and the binding rate constants are the basis for
an extended theoretical model developed and analyzed in an accompanying
report.
