AJP - Gastrointestinal and Liver Physiology, Vol 249, Issue 5 642-G648, Copyright © 1985 by American Physiological Society
Factors influencing triacylglycerol delivery into mesenteric lymph
C. M. Mansbach 2nd, A. Arnold and M. A. Cox
The transport of triacylglycerol (TG) in mesenteric lymph was studied in
rats with duodenal and mesenteric lymphatic cannulas with or without bile
fistulas. Rats were infused with 135 mumol glycerol trioleate (TO) for 4 h,
followed by 5 h of NaCl infusion. Rats with intact fistulas prefed 20% corn
oil had nearly twice the maximum output of TG in lymph as controls. Decay
from peak values was zero order for controls and indeterminate for rats
prefed corn oil. In rats with bile fistulas, less TG was transported in
lymph than in those in which 2 mM phosphatidylcholine (PC) was added to the
infusate. The decay from maximum values was zero order for controls and
first order for rats infused with PC and TO. Recovery of infused
[3H]glycerol trioleate in controls was 43% and increased to 68% on
inclusion of PC in the infusate. We conclude that in chow-fed rats lymph TG
delivery rates were well below infusion rates, suggesting alternate TG
transport routes, TG transport was improved by supplementing the infusate
with PC or prefeeding with 20% TG in chow, and PC may be limiting in TG
transport in rats with bile fistulas.
