AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 252: G209-G214, 1987;
0193-1857/87 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fitzpatrick, L. R.
Right arrow Articles by Johnson, L. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fitzpatrick, L. R.
Right arrow Articles by Johnson, L. R.

AJP - Gastrointestinal and Liver Physiology, Vol 252, Issue 2 209-G214, Copyright © 1987 by American Physiological Society

ARTICLES

Effect of epidermal growth factor on polyamine-synthesizing enzymes in rat enterocytes

L. R. Fitzpatrick, P. Wang and L. R. Johnson

A number of peptides having trophic activity on gastrointestinal mucosa and growth factors are known to induce small intestinal ornithine decarboxylase (ODC) activity. The effect of peptides on ODC and S-adenosylmethionine (SAMDC) activities (key enzymes in polyamine biosynthesis) in isolated enterocytes is unknown. Male Sprague-Dawley rats were fasted for 72 h and injected intraperitoneally with epidermal growth factor (EGF), pentagastrin, or glucagon, or intragastrically with EGF. A similar volume of water served as a control. Villus tip, midvillus, and crypt cell fractions were collected and identified. ODC and SAMDC activities were determined in these cells 4 h after peptide injection. EGF given intraperitoneally, but not intragastrically, stimulated ODC activity along the cryptvillus column. Pentagastrin and glucagon did not induce polyamine biosynthetic enzyme activity. ODC and SAMDC activities in intestinal mucosal scrapings from fasted animals also were increased 2-4 h after intraperitoneal EGF treatment. It is possible that EGF binding at the serosal surface of the crypt enterocyte and subsequent ODC induction is important in initiating the cellular proliferation that is known to occur after treatment with this peptide.


This article has been cited by other articles:


Home page
 IOVSHome page
D. A. Johnson, C. Fields, A. Fallon, M. E. C. Fitzgerald, M. J. Viar, and L. R. Johnson
Polyamine-Dependent Migration of Retinal Pigment Epithelial Cells
Invest. Ophthalmol. Vis. Sci., April 1, 2002; 43(4): 1228 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Banan, J. Z. Fields, Y. Zhang, and A. Keshavarzian
Key role of PKC and Ca2+ in EGF protection of microtubules and intestinal barrier against oxidants
Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G828 - G843.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
R. BAUSKE, V. MILOVIC, L. TURHANOWA, W. F. CASPARY, and J. STEIN
Influence of Epidermal Growth Factor/Transforming Growth Factor Alpha and Polyamines on Caco-2 Cell Proliferation
Ann. N.Y. Acad. Sci., November 17, 1998; 859(1): 198 - 200.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online