AJP - Gastrointestinal and Liver Physiology, Vol 252, Issue 2 276-G280, Copyright © 1987 by American Physiological Society
Rat hepatic bile acid sulfotransferase: enzyme response to androgens and estrogens
R. H. Collins, L. Lack and P. G. Killenberg
Rat liver bile sulfotransferase activity can be divided into a fraction
that reacts with a monoclonal antibody (PK1B) and another fraction that
does not. This work was performed to analyze the known response of hepatic
bile acid sulfotransferase activity to androgens and estrogens by
determining the effect of treatment on the proportion of bile acid
sulfotransferase activity that possessed the epitope for PK1B monoclonal
antibody. Activity in treated animals was further characterized by
high-performance liquid chromatography (HPLC) analysis following
purification by PK1B-immunoadsorption chromatography. The results indicate
that estrogens and androgens affect the subset of enzyme activity that has
the PK1B epitope more than the population that does not. HPLC demonstrates
that increases and decreases in activity that follow treatment with
androgens and estrogens are mirrored by the proportion of the PK1B-reactive
protein that exhibits a relative molecular weight (Mr) greater than
170,000. Radial immunodiffusion assays of hepatic supernatant using a
polyclonal antibody raised against PK1B-reactive bile acid sulfotransferase
show that changes in specific activity that follow treatment are the result
of changes in enzyme protein concentration.
