AJP - Gastrointestinal and Liver Physiology, Vol 254, Issue 2 183-G188, Copyright © 1988 by American Physiological Society
Effect of somatostatin on basal and stimulated gastric secretion in the cod, Gadus morhua
B. Holstein and C. Cederberg
Department of Zoophysiology, University of Goteborg, Sweden.
In vivo secretion of gastric acid and pepsin has been studied in
pylorus-ligated cod. Basal acid output amounted to 100-150 mumol
H+.kg-1.h-1 and pepsin secretion to 1 mg.kg-1.h-1. In response to bombesin
nonapeptide (2.4 nmol.kg-1.h-1) and histamine (81 nmol.kg-1.h-1), acid
secretion increased to approximately 200 and 600% of the basal level,
respectively. Pepsin output was marginally affected by histamine but
increased to approximately 3 and 15 times the basal level during treatment
with bombesin and eledoisin (3.27 nmol.kg-1.h-1). Somatostatin (SS-14, 15
nmol.kg-1.h-1) inhibited basal acid secretion by 85%. It also inhibited the
acid secretion during stimulation with bombesin (68%) and histamine (57%),
but although the former effect could be explained by removal of the basal
component, the latter could not. Basal pepsin secretion was not affected by
SS-14. A slight inhibition (28%) of the peak pepsin response to eledoisin
was demonstrated, and bombesin failed to stimulate pepsin secretion during
treatment with SS-14. These results indicate that endogenous somatostatin,
if present in the cod stomach, could play a role in the regulation of
gastric secretion.
