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Am J Physiol Gastrointest Liver Physiol 254: G483-G488, 1988;
0193-1857/88 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 254, Issue 4 483-G488, Copyright © 1988 by American Physiological Society

ARTICLES

Evidence for the involvement of 5-lipoxygenase products in ethanol-induced intestinal plasma protein loss

I. T. Beck, A. J. Boyd and P. K. Dinda
Department of Medicine, Queen's University, Hotel Dieu Hospital, Ontario, Canada.

In this study we investigated whether the products of 5-lipoxygenase (5-LO) were involved in the jejunal microvascular injury induced by intraluminal ethanol (ETH). A group of rabbits was given orally a selective inhibitor of 5-LO (L-651,392, Merck Frosst Canada) in two 10-mg doses, 24 and 2 h before the experiments (treated group). Another group received no such treatment (untreated group). In each animal of both groups, a jejunal segment was perfused with a control solution (control segment) and an adjacent segment with an ETH-containing (6% wt/vol) solution (ETH-perfused segment). In a series of experiments, we measured 5-LO activity of the jejunal segments of both groups using the generation of leukotriene B4 (LTB4) as an index. In a second series of experiments, we determined the ETH-induced intraluminal protein loss, which was taken as a measure of mucosal microvascular damage. In the untreated group, LTB4 generation (pg/mg tissue) by the ETH-treated segment (2.49 +/- 0.70) was higher (P less than 0.005) than that by the control segment (0.68 +/- 0.14). In the treated group, the LTB4 generation decreased (P less than 0.05) both in the control (0.15 +/- 0.03) and the ETH-perfused segments (0.44 +/- 0.09), but the difference between the two segments still remained significant. ETH caused a 13-fold increase (P less than 0.01) in jejunal protein loss in the untreated group but only a 5-fold increase (P less than 0.05) in the treated group. The ETH-induced increase in protein loss was significantly lower in the treated than in the untreated group (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)


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