AJP - Gastrointestinal and Liver Physiology, Vol 254, Issue 4 575-G579, Copyright © 1988 by American Physiological Society
Phosphorus nuclear magnetic resonance in isolated perfused rat pancreas
T. Matsumoto, T. Kanno, Y. Seo, M. Murakami and H. Watari
Department of Physiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Phosphorus nuclear magnetic resonance spectroscopy was applied to measure
phosphorus energy metabolites in isolated perfused rat pancreas. The gland
was perfused with a modified Krebs-Henseleit solution at room temperature
(25 degrees C). 31P resonances of creatine phosphate (PCr), ATP, ADP,
inorganic phosphate (Pi), and phosphomonoesters (PMEs) were observed in all
the preparations of pancreas. In different individual preparations, the
resonance of PCr varied, but those of ATP were almost the same. The initial
levels of PCr and ATP in individual preparations, however, remained almost
unchanged during perfusion with the standard solution for 2 h. When the
perfusion was stopped, the levels of ATP and PCr decreased, while the
levels of PME and Pi increased. At that time, the Pi resonance shifted to a
higher magnetic field, indicating that the tissue pH decreased. On
reperfusion, the tissue levels of phosphorus compounds and the tissue pH
were restored to their initial resting levels. Continuous infusion of 0.1
microM acetylcholine caused marked and sustained increases in the flow of
pancreatic juice and protein output. During the stimulation the tissue
levels of phosphorus compounds remained unchanged, while the tissue pH was
decreased slightly (0.05 pH unit).
