AJP - Gastrointestinal and Liver Physiology, Vol 255, Issue 1 40-G45, Copyright © 1988 by American Physiological Society
Immunoneutralization of somatostatin and neurotensin: effect on gastric acid secretion
A. Seal, E. Liu, A. Buchan and J. Brown
Department of Surgery, University of British Columbia, Vancouver, Canada.
Cell lines producing monoclonal antibodies to somatostatin and neurotensin,
designated S-10 and NT-C5, respectively, have recently been generated. The
purpose of the present immunoneutralization study in urethan-anesthetized
gastric fistula rats is 1) to examine the ability of these antibodies to
block the inhibitory effect of their target peptides on meal-stimulated
gastric acid secretion and 2) to use these antibodies as probes to
determine whether somatostatin and/or neurotensin are involved in the
inhibition of gastric acid secretion produced by intraduodenal,
intra-ileal, and intracolonic fat infusions. The results demonstrate that
both S-10 and NT-C5 successfully bound exogenous somatostatin and
neurotensin in vivo. S-10 but not NT-C5 prevented the inhibition of gastric
acid secretion produced by intraduodenal fat. NT-C5 but not S-10 prevented
the inhibition of gastric acid secretion produced by intra-ileal fat.
Neither S-10 nor NT-C5 prevented the inhibition of gastric acid secretion
produced by intracolonic fat. We conclude that somatostatin is associated
with proximal and neurotensin with distal small bowel intestinal
fat-induced inhibition of gastric acid secretion.
