AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 255: G462-G469, 1988;
0193-1857/88 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ahmad, S.
Right arrow Articles by Daniel, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ahmad, S.
Right arrow Articles by Daniel, E. E.

AJP - Gastrointestinal and Liver Physiology, Vol 255, Issue 4 462-G469, Copyright © 1988 by American Physiological Society

ARTICLES

[3H]saxitoxin as a marker for canine deep muscular plexus neurons

S. Ahmad, H. D. Allescher, H. Manaka, Y. Manaka and E. E. Daniel
Department of Neurosciences, McMaster University Medical Center, Hamilton, Ontario, Canada.

The objectives of this study are 1) to examine the potential of [3H]saxitoxin binding as a marker for the neuronal membranes in canine small intestinal muscle membrane preparations, 2) to develop a synaptosomal preparation from deep muscular plexus, and 3) to partially characterize [3H]saxitoxin binding to this fraction. A purified synaptosomal fraction, relatively low in the smooth muscle plasma membrane marker enzyme 5'-nucleotidase but enriched in [3H]saxitoxin binding (2,592 fmol/mg), was obtained on sucrose density gradient. Vasoactive intestinal peptide immunoreactivity was also highest (51.82 pmol/mg protein) in this fraction. The binding was rapid at 20 degrees C with quick and complete dissociation after the addition of excess unlabeled tetrodotoxin (TTX). Scatchard analysis of the saturation binding data revealed a single population of binding sites (Bmax = 5,705 fmol/mg protein). The affinity constants calculated from the kinetic and saturation data were in close agreement (Kd = 0.26 and 0.69 nM, respectively). TTX competed for the binding (Ki = 2.1 nM), whereas veratridine and guanidinium hydrochloride did not. Monovalent and divalent cations had differential effects on the binding.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. Miller, A. Roy, S. St-Pierre, M. Dagenais, R. Lapointe, and P. Poitras
Motilin receptors in the human antrum
Am J Physiol Gastrointest Liver Physiol, January 1, 2000; 278(1): G18 - G23.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Kurjak, D. Sattler, V. Schusdziarra, and H. D. Allescher
Characterization of Prejunctional and Postjunctional Muscarinic Receptors of the Ascending Reflex Contraction in Rat Ileum
J. Pharmacol. Exp. Ther., August 1, 1999; 290(2): 893 - 900.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Huber, D. Saur, M. Kurjak, V. Schusdziarra, and H.-D. Allescher
Characterization and splice variants of neuronal nitric oxide synthase in rat small intestine
Am J Physiol Gastrointest Liver Physiol, November 1, 1998; 275(5): G1146 - G1156.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. K. Mao, Y. F. Wang, C. Moogk, J. E. T. Fox-Threlkeld, Q. Xiao, T. J. McDonald, and E. E. Daniel
Locations and molecular forms of PACAP and sites and characteristics of PACAP receptors in canine ileum
Am J Physiol Gastrointest Liver Physiol, January 1, 1998; 274(1): G217 - G225.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online