AJP - Gastrointestinal and Liver Physiology, Vol 255, Issue 6 723-G730, Copyright © 1988 by American Physiological Society
Effect of immunization with prostaglandin metabolites on gastrointestinal ulceration
J. S. Redfern, E. Lee and M. Feldman
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
Active immunization of rabbits with a 6-ketoprostaglandin F1
alpha-thyroglobulin conjugate induced gastrointestinal ulceration, whereas
active immunization of rabbits with 13,14-dihydro-15-keto prostaglandin
E2-thyroglobulin conjugate or with thyroglobulin alone did not result in
ulceration. Passive immunization of a separate group of rabbits with
6-ketoprostaglandin F1 alpha-hyperimmune plasma, obtained from actively
6-ketoprostaglandin F1 alpha-immunized donor rabbits that had ulcers,
induced gastric ulceration within 9 days, whereas passive immunization of
rabbits with control plasma, obtained from donor rabbits actively immunized
with thyroglobulin alone, did not induce ulceration. Ulcerogenic donor
plasma containing antibody to 6-ketoprostaglandin F1 alpha neutralized the
inhibitory actions of prostacyclin on adenosine diphosphate-induced
platelet aggregation, indicating that this antibody cross-reacted with
prostacyclin. In contrast, plasma containing antibodies to
13,14-dihydro-15-ketoprostaglandin E2 cross-reacted only slightly with
prostaglandin E2. Thus antibodies to inactive metabolites of prostaglandins
induce ulceration only if these antibodies cross-react with an endogenous,
"cytoprotective" prostaglandin.
