AJP - Gastrointestinal and Liver Physiology, Vol 256, Issue 1 233-G239, Copyright © 1989 by American Physiological Society

ARTICLES

Characterization of acetylcholine release from enzyme-dissociated myenteric ganglia

W. M. Yau, J. A. Dorsett and E. L. Parr
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901.

Longitudinal muscle strips adhered with myenteric plexus were subjected to enzyme digestion under controlled conditions in a Krebs-bicarbonate buffer solution containing a mixture of collagenase, deoxyribonuclease, protease, choline chloride, and bovine serum albumin for 30 min at 37 degrees C. Myenteric ganglia, singly or in multiple aggregates, were harvested with micropipette and labeled with [3H]choline for [3H]acetylcholine (ACh) release studies. When examined by light or electron (transmission or scanning) microscopy, the ganglia exhibited their normal structural characteristics with axon bundles, dendrites, cell bodies, and vesiculated processes. Depolarization with elevated potassium or veratrine hydrochloride significantly elevated the efflux of [3H] ACh. Perfusion with tachykinins (substance P and substance K), vasoactive intestinal peptide, forskolin, or serotonin also significantly increased the release of [3H]ACh. This study demonstrated that enzyme-dissociated myenteric ganglia, notably free of muscle or connective tissue components, were structurally well preserved and were amenable to functional studies targeted specifically for the enteric plexus neurons.

This article has been cited by other articles:

				G.-D. Wang, X.-Y. Wang, H.-Z. Hu, X.-C. Fang, S. Liu, N. Gao, and Y. Xia Platelet-activating factor in the enteric nervous system of the guinea pig small intestine. Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G928 - G937. [Abstract] [Full Text] [PDF]

				G.-D. Wang, X.-Y. Wang, H.-Z. Hu, X.-C. Fang, S. Liu, N. Gao, Y. Xia, and J. D. Wood Angiotensin receptors and actions in guinea pig enteric nervous system Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G614 - G626. [Abstract] [Full Text] [PDF]

				G. Sarnelli, P. V. Berghe, P. Raeymaekers, J. Janssens, and J. Tack Inhibitory effects of galanin on evoked [Ca2+]i responses in cultured myenteric neurons Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G1009 - G1014. [Abstract] [Full Text] [PDF]

				W.-P. Chen and A. L. Kirchgessner Activation of group II mGlu receptors inhibits voltage-gated Ca2+ currents in myenteric neurons Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1282 - G1289. [Abstract] [Full Text] [PDF]

				M.-t. Liu and A. L. Kirchgessner Agonist- and Reflex-Evoked Internalization of Metabotropic Glutamate Receptor 5 in Enteric Neurons J. Neurosci., May 1, 2000; 20(9): 3200 - 3205. [Abstract] [Full Text] [PDF]

				M. Hanani, M. Francke, W. Hartig, J. Grosche, A. Reichenbach, and T. Pannicke Patch-clamp study of neurons and glial cells in isolated myenteric ganglia Am J Physiol Gastrointest Liver Physiol, April 1, 2000; 278(4): G644 - G651. [Abstract] [Full Text] [PDF]

				J. R. Grider Regulation of excitatory neural input to longitudinal intestinal muscle by myenteric interneurons Am J Physiol Gastrointest Liver Physiol, November 1, 1998; 275(5): G973 - G978. [Abstract] [Full Text] [PDF]

				A. L. Kirchgessner, M.-T. Liu, and F. Alcantara Excitotoxicity in the Enteric Nervous System J. Neurosci., November 15, 1997; 17(22): 8804 - 8816. [Abstract] [Full Text] [PDF]

				Y. Kojima, Y. Tsunoda, and C. Owyang Adenosine 3',5'-Cyclic Monophosphate-Stimulated Ca++ Efflux and Acetylcholine Release in Ileal Myenteric Plexus Are Mediated by N-Type Ca++ Channels: Inhibition by the Kappa Opioid Receptor Agonist J. Pharmacol. Exp. Ther., July 1, 1997; 282(1): 403 - 409. [Abstract] [Full Text]