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Am J Physiol Gastrointest Liver Physiol 256: G299-G305, 1989;
0193-1857/89 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 256, Issue 2 299-G305, Copyright © 1989 by American Physiological Society

ARTICLES

Evidence for a chloride conductance in secretory membrane of parietal cells

A. Perez, D. Blissard, G. Sachs and S. J. Hersey
Department of Physiology, Emory University, Atlanta, Georgia 30322.

A fluorescence-quench method using acridine orange as the probe was employed to monitor acid formation in situ by detergent-permeabilized gastric glands. In KCl medium, the addition of ATP to the permeabilized glands resulted in a rapid decrease in fluorescence and addition of valinomycin resulted in a second phase of fluorescence quench. The fluorescence was restored by addition of the H+-K+-ATPase inhibitor, Sch 28080. An ATP-dependent fluorescence quench was observed also in K2SO4 or K+-isethionate medium; however, valinomycin was ineffective in the Cl-free media. The ATP-dependent quench could be reversed or prevented by the electrogenic protonophore, tetrachlorosalicylanilide (TCS), in KCl medium but not in Cl-free media. The results with TCS are interpreted as demonstrating a large Cl- conductance in the secretory membrane, whereas the results with valinomycin indicate that resting membranes lack a K+ conductance. The data suggest that a complex KCl pathway that may demonstrate a Cl- conductance is used to activate acid secretion.


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D. Heitzmann and R. Warth
No Potassium, No Acid: K+ Channels and Gastric Acid Secretion
Physiology, October 1, 2007; 22(5): 335 - 341.
[Abstract] [Full Text] [PDF]


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