AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 256: G631-G636, 1989;
0193-1857/89 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campbell, V. W.
Right arrow Articles by Yamada, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campbell, V. W.
Right arrow Articles by Yamada, T.

AJP - Gastrointestinal and Liver Physiology, Vol 256, Issue 3 631-G636, Copyright © 1989 by American Physiological Society

ARTICLES

Carbonic anhydrase II gene expression in isolated canine gastric parietal cells

V. W. Campbell, J. Del Valle, M. Hawn, J. Park and T. Yamada
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0682.

The participation of carbonic anhydrase (CA II) in the gastric acid secretory process has been the subject of considerable controversy. We utilized a cDNA probe for CA II to measure CA II gene expression in canine gastric parietal cells that had been stimulated with the three principal acid secretagogues, carbachol, gastrin, and histamine. Hybridization to dot blots of total parietal cell RNA showed a significant increase in specific CA II mRNA content within minutes of secretagogue addition: carbachol stimulation led to an increase of 52 +/- 8.9%, reaching a maximum within 20 min; gastrin stimulation led to an increase within 60 min of 104 +/- 10.6%; stimulation with histamine was followed within 20 min by an increase of 30 +/- 7.2%. We also measured transcription rates for the CA II gene in cells stimulated by each agent and found an increase within 15 min. Our results show that CA II gene expression is regulated by agents that stimulate the parietal cell to secrete acid and that the accumulation of CA II mRNA subsequent to the initial interaction of stimulant appears to result from new transcription of the CA II gene. These data suggest the participation of CA II in the acid secretory response of the parietal cell to secretagogues.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. Pausawasdi, S. Ramamoorthy, L. J. Crofford, F. K. Askari, and A. Todisco
Regulation and function of COX-2 gene expression in isolated gastric parietal cells
Am J Physiol Gastrointest Liver Physiol, June 1, 2002; 282(6): G1069 - G1078.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Nagahara, L. Wang, J. Del Valle, and A. Todisco
Regulation of c-Jun NH2-terminal kinases in isolated canine gastric parietal cells
Am J Physiol Gastrointest Liver Physiol, October 1, 1998; 275(4): G740 - G748.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
I L P Beales and J Calam
Interleukin 1beta and tumour necrosis factor alpha  inhibit acid secretion in cultured rabbit parietal cells by multiple pathways
Gut, February 1, 1998; 42(2): 227 - 234.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Takeuchi, J. Yamada, T. Yamada, and A. Todisco
Functional role of extracellular signal-regulated protein kinases in gastric acid secretion
Am J Physiol Gastrointest Liver Physiol, December 1, 1997; 273(6): G1263 - G1272.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online