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Am J Physiol Gastrointest Liver Physiol 257: G235-G241, 1989;
0193-1857/89 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 257, Issue 2 235-G241, Copyright © 1989 by American Physiological Society

ARTICLES

Somatostatin receptors on canine fundic D-cells: evidence for autocrine regulation of gastric somatostatin

J. Park, T. Chiba, K. Yokotani, J. DelValle and T. Yamada
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109-0362.

Somatostatin is known to have inhibitory effects on the release and action of a wide variety of gut peptides. Previous studies in vivo have suggested a potential inhibitory role for somatostatin even on its own secretion. To determine whether this autoregulatory effect is the result of a direct action of the peptide on the cell that is responsible for its secretion, we examined the effect of a non-immunoreactive but biologically active analogue of somatostatin ([Leu8-D-Trp22-Tyr25]S28) on release of somatostatin-like immunoreactivity (SLI) from isolated canine fundic D-cells. We identified somatostatin binding sites with dissociation constants of 1.2 X 10(-9) and 3.8 X 10(-8) M that coenriched with D-cells. Somatostatin 14, somatostatin 28, and [Leu8-D-Trp22-Tyr25]S28 were equivalent in displacing 125I-[Leu8-D-Trp22-Tyr25]S28 from the binding sites. [Leu8-D-Trp22-Tyr25]S28 inhibited SLI release from D-cells stimulated with (DBcAMP), and pentagastrin. Pertussis toxin pretreatment prevented the inhibitory effects of [Leu8-D-Trp22-Tyr25]S28 on both SLI secretion and cAMP accumulation by on both SLI secretion and cAMP accumulation by D-cells stimulated with epinephrine and forskolin. In contrast, [Leu8-D-Trp22-Tyr25]S28 inhibition of SLI release induced by DBcAMP and pentagastrin was not altered by pertussis toxin. Our data suggest that somatostatin autoregulates its own secretion via specific receptors on D-cells. This inhibitory effect is mediated by mechanisms that are both dependent on and independent of pertussis toxin-sensitive inhibitory guanine nucleotide binding proteins.


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I L P Beales and J Calam
The histamine H3 receptor agonist Nalpha -methylhistamine produced by Helicobacter pylori does not alter somatostatin release from cultured rabbit fundic D-cells
Gut, August 1, 1998; 43(2): 176 - 181.
[Abstract] [Full Text] [PDF]


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