AJP - Gastrointestinal and Liver Physiology, Vol 257, Issue 4 504-G510, Copyright © 1989 by American Physiological Society

ARTICLES

Angiotensin receptors and angiotensin I-converting enzyme in rat intestine

K. A. Duggan, F. A. Mendelsohn and N. R. Levens
Department of Medicine, Austin Hospital, Melbourne, Victoria, Australia.

The purpose of this study was to map the distribution of angiotensin II (ANG II) receptors and ANG I-converting enzyme (ACE) in rat intestine. ANG II binding sites were visualized by in vitro autoradiography using iodinated [Sar1, Ile8]ANG II. The distribution of ACE was mapped using an iodinated derivative of lisinopril. Male Sprague-Dawley rats were killed and the interior of the whole intestine washed with ice-cold saline. Segments of duodenum, jejunum, ileum, and colon were quickly frozen in a mixture of isopentane and dry ice. Twenty-micron frozen sections were thaw-mounted onto gelatin-coated slides, incubated with either ligand, and exposed to X-ray film. After exposure and subsequent development, the films were quantitated by computerized densitometry. ANG II receptors were most dense in the colon, followed by the ileum, duodenum, and jejunum. Within each segment of intestine, specific ANG II binding sites were localized exclusively to the muscularis. In contrast, ACE was present in both the mucosa and the muscularis. The colocalization of ANG II receptors and ACE may suggest a role for locally generated ANG II in the control of intestinal function. The luminal orientation of ACE in the mucosa of the small intestine may suggest that at this site ACE serves primarily to hydrolyze dietary peptides.

This article has been cited by other articles:

				M. Paul, A. Poyan Mehr, and R. Kreutz Physiology of local Renin-Angiotensin systems. Physiol Rev, July 1, 2006; 86(3): 747 - 803. [Abstract] [Full Text] [PDF]

				M. A. F. De Godoy and S. Rattan Autocrine regulation of internal anal sphincter tone by renin-angiotensin system: comparison with phasic smooth muscle Am J Physiol Gastrointest Liver Physiol, December 1, 2005; 289(6): G1164 - G1175. [Abstract] [Full Text] [PDF]

				G.-D. Wang, X.-Y. Wang, H.-Z. Hu, X.-C. Fang, S. Liu, N. Gao, Y. Xia, and J. D. Wood Angiotensin receptors and actions in guinea pig enteric nervous system Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G614 - G626. [Abstract] [Full Text] [PDF]

				B. Johansson, M. Holm, S. Ewert, A. Casselbrant, A. Pettersson, and L. Fandriks Angiotensin II type 2 receptor-mediated duodenal mucosal alkaline secretion in the rat Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1254 - G1260. [Abstract] [Full Text] [PDF]

				Y. Hosoda, A. Winarto, T. Iwanaga, and A. Kuwahara Mode of action of ANG II on ion transport in guinea pig distal colon Am J Physiol Gastrointest Liver Physiol, April 1, 2000; 278(4): G625 - G634. [Abstract] [Full Text] [PDF]

				X.-H. Jin, Z.-Q. Wang, H. M. Siragy, R. L. Guerrant, and R. M. Carey Regulation of jejunal sodium and water absorption by angiotensin subtype receptors Am J Physiol Regulatory Integrative Comp Physiol, August 1, 1998; 275(2): R515 - R523. [Abstract] [Full Text] [PDF]

				M. Hatch, R. W. Freel, and N. D. Vaziri Local upregulation of colonic angiotensin II receptors enhances potassium excretion in chronic renal failure Am J Physiol Renal Physiol, February 1, 1998; 274(2): F275 - F282. [Abstract] [Full Text] [PDF]