AJP - Gastrointestinal and Liver Physiology, Vol 257, Issue 4 532-G538, Copyright © 1989 by American Physiological Society

ARTICLES

Putative mechanisms involved in excitatory and inhibitory effects of somatostatin on intestinal motility

T. Takeda, K. Taniyama, S. Baba and C. Tanaka
Department of Pharmacology, Kobe University School of Medicine, Japan.

The mechanism of action of somatostatin on the motility of intestine was examined in the entire preparation and the longitudinal muscle attached with Auerbach's plexus (LA) preparation of guinea pig ileum, in relation to the cholinergic neuron and gamma-aminobutyric acid (GABA)ergic neuron. Somatostatin produced a transient potentiation of electrical stimulation-induced twitch contractions followed by an inhibition. The excitatory effect of somatostatin was associated with an increase in the release of [3H]acetylcholine (ACh) from the preparations preloaded with [3H]choline. Bicuculline, a GABAA antagonist, inhibited the somatostatin-induced excitatory effect. Somatostatin inhibited the electrical stimulation-induced twitch contraction and release of [3H]ACh, and the inhibition was greater in the entire preparation than in the LA. Phaclofen, a GABAB antagonist, prevented the inhibitory effects of somatostatin. Somatostatin induced a Ca2+ -dependent, tetrodotoxin-sensitive release of [3H]GABA from the preparations preloaded with [3H]GABA. Therefore somatostatin exerts excitatory and inhibitory effects on the cholinergic neuron due to the stimulation of the GABAergic neuron, and the motility of the intestine is regulated.

This article has been cited by other articles:

				J. R. Grider Regulation of excitatory neural input to longitudinal intestinal muscle by myenteric interneurons Am J Physiol Gastrointest Liver Physiol, November 1, 1998; 275(5): G973 - G978. [Abstract] [Full Text] [PDF]