AJP - Gastrointestinal and Liver Physiology, Vol 257, Issue 5 845-G850, Copyright © 1989 by American Physiological Society

ARTICLES

Effects of platelet-activating factor on ion transport in isolated rat jejunum

A. C. Hanglow, J. Bienenstock and M. H. Perdue
Intestinal Disease Research Unit, McMaster University, Hamilton, Ontario, Canada.

In isolated normal rat jejunum, platelet-activating factor (PAF) induced a dose-dependent increase in short-circuit current (Isc) that was reduced in chloride-free buffer and inhibited by the Cl- channel blocker, diphenylamine-2-carboxylate. An immediate rise in Isc (early phase) occurred that fell to a new elevated base line by 15 min (late phase). These responses to PAF occurred only when experiments were conducted at or before approximately 9 A.M. Early phase responses were blocked by the specific PAF antagonists, BN52021 and WEB2086, and were inhibited by the neurotoxin, tetrodotoxin. Early and late phases were also reduced by cyclooxygenase inhibitors and by doxantrazole, a mast cell stabilizing drug. However, histamine and serotonin antagonists were ineffective. We conclude that PAF causes changes in ion transport that include Cl- secretion and acts on the epithelium possibly via an intermediate cell and enteric nerves. In addition, known PAF receptors are involved in one component of the response that appears to follow a circadian rhythm.

This article has been cited by other articles:

				E. C. Claud, J. Lu, X. Q. Wang, M. Abe, E. O. Petrof, J. Sun, D. J. Nelson, J. Marks, and T. Jilling Platelet-activating factor-induced chloride channel activation is associated with intracellular acidosis and apoptosis of intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, May 1, 2008; 294(5): G1191 - G1200. [Abstract] [Full Text] [PDF]

				G.-D. Wang, X.-Y. Wang, H.-Z. Hu, X.-C. Fang, S. Liu, N. Gao, and Y. Xia Platelet-activating factor in the enteric nervous system of the guinea pig small intestine. Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G928 - G937. [Abstract] [Full Text] [PDF]

				J. L. Wallace and L. Ma Inflammatory Mediators in Gastrointestinal Defense and Injury Experimental Biology and Medicine, December 1, 2001; 226(11): 1003 - 1015. [Abstract] [Full Text] [PDF]

				C J Bell, E J Elliott, J L Wallace, D M Redmond, J Payne, Z Li, and E V O'Loughlin Do eicosanoids cause colonic dysfunction in experimental E coli 0157:H7 (EHEC) infection? Gut, June 1, 2000; 46(6): 806 - 812. [Abstract] [Full Text] [PDF]

				M. F. G. Rocha, A. M. Soares, C. A. Flores, T. S. Steiner, D. M. Lyerly, R. L. Guerrant, R. A. Ribeiro, and A. A. M. Lima Intestinal Secretory Factor Released by Macrophages Stimulated with Clostridium difficile Toxin A: Role of Interleukin 1beta Infect. Immun., October 1, 1998; 66(10): 4910 - 4916. [Abstract] [Full Text] [PDF]

				T. A. Hinterleitner, J. D. Valentich, J.-H. Cha, P. Will, A. Welton, and D. W. Powell J. Pharmacol. Exp. Ther., June 1, 1997; 281(3): 1264 - 1271. [Abstract] [Full Text]