AJP - Gastrointestinal and Liver Physiology, Vol 258, Issue 3 338-G343, Copyright © 1990 by American Physiological Society

ARTICLES

Mechanical properties of isolated human esophageal smooth muscle

A. Tottrup, A. Forman, N. Uldbjerg, P. Funch-Jensen and K. E. Andersson
Department of Surgical Gastroenterology L, Aarhus Municipal Hospital, Denmark.

Isolated smooth muscle strips from the human esophagus representing both the longitudinal and circular layers of the esophagogastric junction and the esophageal body were prepared. The strips were mounted in organ baths, and resting length was defined. By repeatedly increasing the length of the strips with 20% of resting length and recording values of resting and active tensions, length-tension relations for each muscle type were constructed. Only circular strips from the esophagogastric junction developed active, resting tension, disclosed by replacing the normal Ca2(+)-containing Krebs solution with Ca2(+)-free medium. Carbachol (10(-6) M) was used for submaximal activation of the contractile apparatus. At lengths between 180 and 260% of resting length, all strips reached optimum length (LO) where further elongation gave no further increase in active tension development. Repeated stimulations with carbachol was possible at a length of 200% of LO without affecting reproducibility. Determination of different collagen components revealed no differences between muscle types.

This article has been cited by other articles:

				S. K. Ghosh, P. J. Kahrilas, and J. G. Brasseur Liquid in the gastroesophageal segment promotes reflux, but compliance does not: a mathematical modeling study Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G920 - G933. [Abstract] [Full Text] [PDF]

				S. M. Sims, T. Chrones, and H. G. Preiksaitis Calcium Sensitization in Human Esophageal Muscle: Role for RhoA Kinase in Maintenance of Lower Esophageal Sphincter Tone J. Pharmacol. Exp. Ther., October 1, 2008; 327(1): 178 - 186. [Abstract] [Full Text] [PDF]

				H. Gregersen, T. Hausken, J. Yang, S. Odegaard, and O. H. Gilja Mechanosensory properties in the human gastric antrum evaluated using B-mode ultrasonography during volume-controlled antral distension Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G876 - G882. [Abstract] [Full Text] [PDF]

				J. R. Kovac, H. G. Preiksaitis, and S. M. Sims Functional and molecular analysis of L-type calcium channels in human esophagus and lower esophageal sphincter smooth muscle Am J Physiol Gastrointest Liver Physiol, December 1, 2005; 289(6): G998 - G1006. [Abstract] [Full Text] [PDF]

				A. Muinuddin, Y. Kang, H. Y. Gaisano, and N. E. Diamant Regional differences in L-type Ca2+ channel expression in feline lower esophageal sphincter Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G772 - G781. [Abstract] [Full Text] [PDF]

				A. A. Gonzalez, R. Farre, and P. Clave Different responsiveness of excitatory and inhibitory enteric motor neurons in the human esophagus to electrical field stimulation and to nicotine Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G299 - G306. [Abstract] [Full Text] [PDF]

				A. Muinuddin, L. Neshatian, H. Y. Gaisano, and N. E. Diamant Calcium source diversity in feline lower esophageal sphincter circular and sling muscle Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G271 - G277. [Abstract] [Full Text] [PDF]

				J. Wang, L. G. Laurier, S. M. Sims, and H. G. Preiksaitis Enhanced capacitative calcium entry and TRPC channel gene expression in human LES smooth muscle Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1074 - G1083. [Abstract] [Full Text] [PDF]

				C. Gao, L. Arendt-Nielsen, W. Liu, P. Petersen, A. M. Drewes, and H. Gregersen Sensory and biomechanical responses to ramp-controlled distension of the human duodenum Am J Physiol Gastrointest Liver Physiol, March 1, 2003; 284(3): G461 - G471. [Abstract] [Full Text] [PDF]

				A. Muinuddin, S. Xue, and N. E. Diamant Regional differences in the response of feline esophageal smooth muscle to stretch and cholinergic stimulation Am J Physiol Gastrointest Liver Physiol, December 1, 2001; 281(6): G1460 - G1467. [Abstract] [Full Text] [PDF]

				S D Smid and L A Blackshaw Neuromuscular function of the human lower oesophageal sphincter in reflux disease and Barrett's oesophagus Gut, June 1, 2000; 46(6): 756 - 761. [Abstract] [Full Text] [PDF]

				A. M. F. Salapatek, A. Lam, and E. E. Daniel Calcium Source Diversity in Canine Lower Esophageal Sphincter Muscle J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 98 - 106. [Abstract] [Full Text]