AJP - GI Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 261: G65-G70, 1991;
0193-1857/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mutoh, H.
Right arrow Articles by Sugimoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mutoh, H.
Right arrow Articles by Sugimoto, T.

AJP - Gastrointestinal and Liver Physiology, Vol 261, Issue 1 65-G70, Copyright © 1991 by American Physiological Society

ARTICLES

Reduced glutathione protects cultured gastric mucosal cells from suckling rats against acid

H. Mutoh, S. Ota, H. Hiraishi, K. J. Ivey, A. Terano and T. Sugimoto
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

We examined the role of reduced glutathione as a defense mechanism against acid-induced gastric mucosal cell damage in vitro. Cellular stores of reduced glutathione were depleted by reaction with diethyl maleate (DEM) or 1-chloro-2,4-dinitrobenzene (CDNB) and increased by reaction with L-cysteine. Depletion of cellular glutathione by reaction with DEM or CDNB potentiated gastric mucosal cell lysis by acid. Increase of cellular glutathione by L-cysteine decreased cell lysis by acid. Altering the cellular reduced-to-oxidized glutathione ratio by tert-butyl hydroperoxide or diamide increased cellular susceptibility to acid. Reduced glutathione is essential for glutathione peroxidase to catalyze hydrogen peroxide. We further studied whether oxygen free radicals were involved in the pathogenesis of acid-induced gastric mucosal injury in vitro. Neither superoxide dismutase, catalase, nor dimethyl sulfoxide decreased acid-induced gastric mucosal cell damage. We conclude that reduced glutathione plays an important role as a defense mechanism against acid-induced injury in cultured rat gastric mucosal cells. Production of oxygen radical in response to acid exposure may occur intracellularly, since exogenous oxygen radical scavengers, which do not gain access to the interior of cells, had no protective effect. Reduced glutathione might protect gastric mucosal cells by mechanisms other than the elimination of oxygen free radicals.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
B. Liu and Y. A. Hannun
Inhibition of the Neutral Magnesium-dependent Sphingomyelinase by Glutathione
J. Biol. Chem., June 27, 1997; 272(26): 16281 - 16287.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online