AJP - Gastrointestinal and Liver Physiology, Vol 261, Issue 2 185-G190, Copyright © 1991 by American Physiological Society

ARTICLES

Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells

Z. Spolarics, C. H. Lang, G. J. Bagby and J. J. Spitzer
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.

This study assessed and compared the rate of glucose utilization, activity of the hexose-monophosphate shunt (HMS), and the oxidation of glutamine, lactate, and palmitate in Kupffer (KC), endothelial (EC), and parenchymal liver cells (PC). Cells were isolated by collagenase and pronase digestion followed by centrifugal elutriation. The freshly isolated cells were incubated in the presence of 5 mM glucose, 0.5 mM glutamine, 1 mM lactate, and 0.4 mM palmitate, and the oxidation rate of individual substrates was determined by the measurement of 14CO2 production. Glucose utilization was assessed by detritiation of [2-3H]glucose. Glucose flux through HMS was 2.6, 1.6, and 0.72 nmol.h-1.mg protein-1 in KC, EC and PC, respectively. The oxidation rate of palmitate in PC (3.5 nmol.h-1.mg protein-1) was about twofold greater than in nonparenchymal cells. Glutamine oxidation was 6.1, 4.2, and 2.1 nmol.h-1.mg protein-1 in KC, EC, and PC, respectively. In contrast, oxidation of exogenous lactate by PC (32.1 nmol.h-1.mg protein-1) was about seven- to eightfold greater than by KC or EC. Presence of prevailing lactate concentrations did not inhibit glucose oxidation in these cells, while it attenuated glucose utilization by PC. Our data show that in the presence of a physiological substrate mixture, less than 20% of the ATP generated from exogenous substrates is derived from glycolysis in KC or EC. Oxidation of glutamine and palmitate are the main sources for energy in these cells. In PC, however, lactate and palmitate oxidation is responsible for approximately 90% for the ATP production derived form the oxidation of exogenous substrates.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				G. I. Nedredal, K. Elvevold, L. M. Ytrebo, O.-M. Fuskevag, I. Pettersen, K. Bertheussen, B. Langbakk, B. Smedsrod, and A. Revhaug Significant contribution of liver nonparenchymal cells to metabolism of ammonia and lactate and cocultivation augments the functions of a bioartificial liver Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G75 - G83. [Abstract] [Full Text] [PDF]

				G. E. Mann, D. L. Yudilevich, and L. Sobrevia Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells Physiol Rev, January 1, 2003; 83(1): 183 - 252. [Abstract] [Full Text] [PDF]

				R. Lohmann, W. W. Souba, and B. P. Bode Rat liver endothelial cell glutamine transporter and glutaminase expression contrast with parenchymal cells Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G743 - G750. [Abstract] [Full Text] [PDF]

				C. M. PASTOR, D. R. MOREL, and T. R. BILLIAR Oxygen Supply Dependence of Urea Production in the Isolated Perfused Rat Liver Am. J. Respir. Crit. Care Med., March 1, 1998; 157(3): 796 - 802. [Abstract] [Full Text] [PDF]