AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 262: G719-G726, 1992;
0193-1857/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cher, D. J.
Right arrow Articles by Jamieson, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cher, D. J.
Right arrow Articles by Jamieson, J. D.

AJP - Gastrointestinal and Liver Physiology, Vol 262, Issue 4 719-G726, Copyright © 1992 by American Physiological Society

ARTICLES

Amylase release from streptolysin O permeabilized fetal pancreatic acini

D. J. Cher, P. J. Padfield and J. D. Jamieson
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.

Developmental regulation of Ca(2+)-dependent protein discharge was investigated in fetal and neonatal rat pancreatic acini permeabilized with streptolysin O. When incubated at 37 degrees C in a Ca(2+)-ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid/K glutamate buffer, permeabilized day 19 and 20 fetal acini demonstrated Ca(2+)-dependent release of amylase, whereas day 21 (term) fetal acini did not. Ca(2+)-dependent amylase release reappeared in day 1, 2, and 6 neonatal pancreases. ATP depletion completely inhibited Ca(2+)-stimulated amylase release from both day 20 fetal and adult acini. Ca(2+)-dependent amylase discharge from day 20 fetal acini was enhanced by the nonhydrolyzable GTP analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), and by the phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA). Ca(2+)-independent GTP gamma S-stimulated amylase release was observed from adult but not from day 20 fetal acini. In contrast to its stimulatory effects in permeabilized adult acini, adenosine 3',5'-cyclic monophosphate (cAMP) alone had little effect on release from permeabilized day 20 fetal acini. Our studies indicate that the fetal pancreas is competent to undergo Ca(2+)-dependent protein secretion but that this secretion is suppressed at birth. Our studies also suggest that the fetal gland is sensitive to modulators of exocytosis active in the adult pancreas, such as GTP gamma S, TPA, and cAMP but responds differently to these agents compared with responses in adult glands.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
R. D. Burgoyne and A. Morgan
Secretory Granule Exocytosis
Physiol Rev, April 1, 2003; 83(2): 581 - 632.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online