AJP - Gastrointestinal and Liver Physiology, Vol 262, Issue 5 779-G784, Copyright © 1992 by American Physiological Society
Gastric inhibitory polypeptide stimulates pancreatic lipase and colipase synthesis in rats
R. D. Duan and C. Erlanson-Albertsson
Department of Medical and Physiological Chemistry, University of Lund, Sweden.
Effects of short-term infusion and long-term injection of gastric
inhibitory polypeptide (GIP) on changes in pancreatic lipase and colipase
contents in rats were studied, and mRNAs encoding for lipase and colipase
were determined by Northern blot hybridization with specific cDNA probes.
GIP infused at a dose of 3 micrograms/h for 24 h significantly increased
the pancreatic lipase content by 34% (P less than 0.05) but had no
significant effect on colipase and amylase contents. No change in mRNAs
encoding for these proteins was found after infusion of GIP for 24 h.
Injection of GIP (5-60 micrograms/kg) three times a day for 5 days dose
dependently increased the contents of lipase and colipase, with the
increase in colipase being more prominent. Injection of GIP for 5 days at a
dose of 30 micrograms.kg-1.day-1 increased colipase and lipase contents by
52 and 25%, and their corresponding mRNAs by 60 and 160%, respectively. The
amylase mRNA was not changed by injection of GIP. It is concluded that GIP
has a specific stimulatory effect on the synthesis of pancreatic lipase and
colipase at both pretranslational and translational levels.
