AJP - Gastrointestinal and Liver Physiology, Vol 262, Issue 6 971-G976, Copyright © 1992 by American Physiological Society

ARTICLES

Dietary regulation of pancreatic amylase in transgenic mice mediated by a 126-base pair DNA fragment

R. M. Schmid and M. H. Meisler
Department of Human Genetics, University of Michigan, Ann Arbor 48109-0618.

Expression of the mouse pancreatic amylase gene Amy-2.2 is increased approximately 10-fold in response to increasing the carbohydrate content of the diet from 9.6 to 74%. The DNA sequence mediating this response has been localized to the 5' flanking region of the amylase gene by analysis of hybrid constructs in transgenic mice. The results define a 127-base pair dietary response unit that includes two previously described regulatory elements, an insulin-responsive element and a pancreatic enhancer. Fragments containing these two elements alone fail to respond to diet, demonstrating a requirement for additional regulatory sequences. Another mouse amylase gene Amy-2.1 is only minimally responsive to insulin and to diet. The data are consistent with the hypothesis that the insulin-response element is necessary but not sufficient for regulation of amylase by dietary carbohydrate.

This article has been cited by other articles:

				K. C. Swanson, J. C. Matthews, A. D. Matthews, J. A. Howell, C. J. Richards, and D. L. Harmon Dietary Carbohydrate Source and Energy Intake Influence the Expression of Pancreatic {alpha}-Amylase in Lambs J. Nutr., September 1, 2000; 130(9): 2157 - 2165. [Abstract] [Full Text]

				K. A. Lacourse, L. J. Swanberg, P. J. Gillespie, J. F. Rehfeld, T. L. Saunders, and L. C. Samuelson Pancreatic function in CCK-deficient mice: adaptation to dietary protein does not require CCK Am J Physiol Gastrointest Liver Physiol, May 1, 1999; 276(5): G1302 - G1309. [Abstract] [Full Text] [PDF]