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Am J Physiol Gastrointest Liver Physiol 263: G908-G912, 1992;
0193-1857/92 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 263, Issue 6 908-G912, Copyright © 1992 by American Physiological Society

ARTICLES

Somatostatin restraint of gastrin secretion in pigs revealed by monoclonal antibody immunoneutralization

J. J. Holst, P. N. Jorgensen, T. N. Rasmussen and P. Schmidt
Department of Medical Physiology C, Panum Institute, Copenhagen, Denmark.

We studied the functional coupling between antral somatostatin and gastrin cells in isolated perfused porcine antrum using immunoneutralization with monoclonal antibodies against somatostatin. Their binding affinity was 10(11) l/mol, and the final binding capacity was 11.7 nmol/ml. Antibody infusion within 1 min increased gastrin secretion, reaching a rate of 349 +/- 64% (means +/- SE, n = 7) of basal secretion (59 +/- 5 pmol/l) after 5 min. The effect of somatostatin at 10(-9) mol/l, which inhibited gastrin secretion from 58 +/- 11 to 14 +/- 3 pmol/min (n = 4), was abolished by antibody infusion. Electrical stimulation of the vagus nerves (n = 7) performed during antibody infusion increased gastrin secretion from 224 +/- 61 to 328 +/- 55 pmol/min, not significantly different from the increase in control experiments from 43 +/- 9 to 118 +/- 20 pmol/min, indicating that the vagal stimulation of gastrin secretion does not depend on mechanisms involving somatostatin. We conclude that paracrine antral somatostatin secretion is one of the most important factors regulating basal gastrin secretion in pigs.


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