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Am J Physiol Gastrointest Liver Physiol 267: G201-G204, 1994;
0193-1857/94 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 267, Issue 2 201-G204, Copyright © 1994 by American Physiological Society

ARTICLES

Effect of age on the expression of phosphoenolpyruvate carboxykinase in rat liver

T. Wimonwatwatee, A. R. Heydari, W. T. Wu and A. Richardson
Department of Chemistry, Illinois State University, Normal 61761.

The effect of age on the expression of phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) was studied using hepatocytes isolated from 6-, 15-, and 26-mo-old-male Fischer 344 rats fasted for 12 h. The activity of PEPCK in hepatocyte extracts decreased 40-50% (P < 0.05) between 6 and 26 mo of age. The age-related decrease in PEPCK activity was paralleled by a similar decrease (P < 0.05) in the level of PEPCK mRNA. Thus the decrease in PEPCK activity appears to arise from a decrease in level of the PEPCK transcript available for translation. The stability, i.e., half-life of PEPCK mRNA in hepatocytes isolated from 6- and 26-mo-old rats was similar; however, the nuclear transcription of PEPCK by hepatocytes isolated from 26-mo-old rats was 44% lower (P < 0.05) than the nuclear transcription of PEPCK by hepatocytes isolated from 6-mo-old rats. Thus the age-related decline in PEPCK mRNA levels in the liver of fasted rats appears to arise from a decrease in the transcription of PEPCK. A significant decrease (P < 0.05) in the activity and mRNA level of PEPCK also was observed with age in kidney tissue isolated from rats fasted for 12 h.


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