AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 267: G322-G327, 1994;
0193-1857/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grider, J. R.
Right arrow Articles by Bonilla, O. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grider, J. R.
Right arrow Articles by Bonilla, O. M.

AJP - Gastrointestinal and Liver Physiology, Vol 267, Issue 2 322-G327, Copyright © 1994 by American Physiological Society

ARTICLES

Differential expression of substance P, somatostatin, and VIP in neurons from cultured myenteric ganglia

J. R. Grider and O. M. Bonilla
Department of Physiology, Medical College of Virginia, Richmond 23298.

The expression of three enteric neuropeptides was examined in freshly dispersed ganglia and in ganglia cultured for up to 28 days. During culture, glial cells grew into a flat sheet surrounding a cluster of neurons identified with neuron-specific enolase (13 +/- 2/ganglion), which remained constant throughout the period of culture. The neurons underwent a distinctive temporal change, resulting in overexpression of substance P (SP), normal expression of somatostatin, and virtual suppression of vasoactive intestinal peptide (VIP). Three weeks after the start of culture, the ganglia contained and released (in response to 55 nM KCl, 0.1 mM 1,1-dimethyl-4-phenylpiperazinium, or 1 microM gastrin-releasing peptide) twice as much SP as freshly dispersed ganglia, corresponding to a sevenfold increase per cultured neuron; content and release of somatostatin did not change. SP content and release declined to 1.5% of those found in control cultures when nonneuronal cells were suppressed with cytosine arabinoside but were partially restored (13-17% of control) by nerve growth factor. In marked contrast, VIP was minimally (< 1%) present in and released from ganglia after the third day in culture. Suppression of VIP could reflect a selective loss of VIP neurons and/or VIP expression.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. A. Nankervis, D. J. Dunaway, and P. T. Nowicki
Determinants of terminal mesenteric artery resistance during the first postnatal month
Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G678 - G686.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Hanani, M. Francke, W. Hartig, J. Grosche, A. Reichenbach, and T. Pannicke
Patch-clamp study of neurons and glial cells in isolated myenteric ganglia
Am J Physiol Gastrointest Liver Physiol, April 1, 2000; 278(4): G644 - G651.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. T. Nowicki
Postnatal changes in gut hemodynamics: a possible role for substance P
Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G1142 - G1150.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online