AJP - Gastrointestinal and Liver Physiology, Vol 267, Issue 6 1108-G1121, Copyright © 1994 by American Physiological Society

ARTICLES

Regional differences in glycoconjugates of intestinal M cells in mice: potential targets for mucosal vaccines

P. J. Giannasca, K. T. Giannasca, P. Falk, J. I. Gordon and M. R. Neutra
Gastrointestinal Cell Biology Laboratory, Children's Hospital, Boston, Massachusetts.

We have used a panel of lectins and antibodies to describe the composition of complex carbohydrates associated with M cells in various regions of the intestinal tract of adult BALB/c mice. The fucose-specific lectin Ulex europaeus agglutinin type I (UEA I) is a marker of M cells in the small intestine and recognized M cells at an early stage of differentiation. Subpopulations of M cells in a single follicle-associated epithelium (FAE) could be distinguished by different fucose-specific probes. Certain lectins revealed that M cells have basal processes that extend into the underlying lymphoid tissue. Colonic and rectal M cells display glycosylation patterns distinct from M cells of Peyer's patches and are characterized by terminal galactose. UEA I selectively adhered to Peyer's patch M cells in mucosal explants and in ligated intestinal loops in vivo. The lectin was taken up into endocytic vesicles and transported to the intra-epithelial pocket and other domains of the basolateral membrane. Thus M cell-specific glycoconjugates could serve as "receptors" for targeting of lectin-antigen conjugates to the mucosal immune system.

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