AJP - GI AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 268: G797-G805, 1995;
0193-1857/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boelsterli, U. A.
Right arrow Articles by Meier, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boelsterli, U. A.
Right arrow Articles by Meier, P. J.

AJP - Gastrointestinal and Liver Physiology, Vol 268, Issue 5 797-G805, Copyright © 1995 by American Physiological Society

ARTICLES

Identification and characterization of a basolateral dicarboxylate/cholate antiport system in rat hepatocytes

U. A. Boelsterli, B. Zimmerli and P. J. Meier
Department of Internal Medicine, University Hospital, Zurich, Switzerland.

The mechanisms and driving forces for the uptake of the unconjugated bile acid cholate were investigated both in cultured rat hepatocytes and in rat liver basolateral (sinusoidal) plasma membrane (BLPM) vesicles. Determination of initial uptake rates of [3H]cholate (0.1 microM) into cultured hepatocytes confirmed that the majority (75%) of the transmembrane transport was mediated by Na(+)-independent mechanisms. This portion of cholate uptake consisted of a pH-sensitive moiety representing nonionic diffusion, which may become quantitatively important at low pH and high cholate concentrations, as well as of a saturable (Michaelis constant 7.4 microM), 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-sensitive transport moiety, suggesting the involvement of a carrier. This latter transport system was functionally characterized by 1) inhibition of cellular cholate uptake in the absence of extracellular sodium by the dicarboxylic acid alpha-ketoglutarate (alpha-KG; 1 mM) and by the organic anion p-aminohippurate (PAH; 1 mM); 2) stimulation of cellular cholate uptake by alpha-KG (10 microM) or PAH (1 mM) in the presence of an inwardly directed sodium gradient; 3) lack of sensitivity toward lithium in BLPM vesicles; 4) trans-stimulation of vesicular cholate uptake by alpha-KG or PAH, but not by benzoate; and 5) cis-inhibition of alpha-KG/alpha-KG self-exchange by extravesicular cholate (400 microM), PAH (5 mM), probenecid, or DIDS. Collectively, these data indicate the presence of a Na(+)-dicarboxylate cotransport-coupled organic anion exchanger in the hepatocyte basolateral plasma membrane that may be involved in cholate uptake in the liver.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
A. Bertuzzi, G. Mingrone, A. Gandolfi, A. V. Greco, and S. Salinari
Disposition of Dodecanedioic Acid in Humans
J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 846 - 852.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
U. Eckhardt, A. Schroeder, B. Stieger, M. Hochli, L. Landmann, R. Tynes, P. J. Meier, and B. Hagenbuch
Polyspecific substrate uptake by the hepatic organic anion transporter Oatp1 in stably transfected CHO cells
Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G1037 - G1042.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sekine, N. Watanabe, M. Hosoyamada, Y. Kanai, and H. Endou
Expression Cloning and Characterization of a Novel Multispecific Organic Anion Transporter
J. Biol. Chem., July 25, 1997; 272(30): 18526 - 18529.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online