| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Gastrointestinal and Liver Physiology, Vol 269, Issue 4 591-G599, Copyright © 1995 by American Physiological Society
ARTICLES |
H. M. Kandil, R. A. Argenzio, W. Chen, H. M. Berschneider, A. D. Stiles, J. K. Westwick, R. A. Rippe, D. A. Brenner and J. M. Rhoads
Department of Pediatrics, University of North Carolina, Chapel Hill 27599-7220, USA.
We studied the effect of L-glutamine (Gln), the principal intestinal fuel, on proliferation of a porcine jejunal cell line, IPEC-J2. In cells synchronized by serum deprivation for 4 h, Gln stimulated ornithine decarboxylase (ODC; EC 4.1.1.17) in a dose- and time-dependent manner, with maximal effects at 10 mM in 3 h (P < 0.01). Similar effects were seen for the structurally related amino acid L-asparagine and serum. The Gln effect on ODC was specific, as isosmolar mannitol, glucose, methyl-beta-D-glucoside, L-phenylalanine, ammonia, and aminoisobutyric acid were ineffective. The alanine aminotransferase inhibitor aminooxyacetate (AO) inhibited the ODC stimulation by Gln in a dose-dependent manner (half-maximal inhibitory concentration = 0.5 mM). AO was not toxic to cells, as determined by propidium iodide uptake into nuclei. In addition, Gln stimulated a twofold increase of cellular 24-h [3H]thymidine incorporation above rates of control cells bathed in standard media (P < 0.01); this effect was also blocked by AO. Gln and phorbol 12-myristate 13-acetate stimulated ODC in a synergistic manner. The Na+/H+ exchange inhibitor methylisobutyl amiloride blocked the enhancement of ODC by Gln. Gln also induced the mRNA of the immediate-early gene c-jun. Gln stimulates proliferation in a porcine jejunal cell line through a mechanism requiring transamination and intact Na+/H+ exchange. This stimulation of enterocyte proliferation by Gln suggests that therapeutic Gln administration could facilitate epithelial recovery in the injured small intestine.
This article has been cited by other articles:
|
|
 |
|
  A. T. Blikslager, A. J. Moeser, J. L. Gookin, S. L. Jones, and J. Odle Restoration of Barrier Function in Injured Intestinal Mucosa Physiol Rev, April 1, 2007; 87(2): 545 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E C Clark, S D Patel, P R Chadwick, G Warhurst, A Curry, and G L Carlson Glutamine deprivation facilitates tumour necrosis factor induced bacterial translocation in Caco-2 cells by depletion of enterocyte fuel substrate Gut, February 1, 2003; 52(2): 224 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wu, N. E. Flynn, and D. A. Knabe Enhanced intestinal synthesis of polyamines from proline in cortisol-treated piglets Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E395 - E402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wu, N. E. Flynn, D. A. Knabe, and L. A. Jaeger A cortisol surge mediates the enhanced polyamine synthesis in porcine enterocytes during weaning Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R554 - R559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G Burrin, B. Stoll, R. Jiang, X. Chang, B. Hartmann, J. J. Holst, G. H Greeley Jr, and P. J Reeds Minimal enteral nutrient requirements for intestinal growth in neonatal piglets: how much is enough? Am. J. Clinical Nutrition, June 1, 2000; 71(6): 1603 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. A. J. Adegoke, M. I. McBurney, S. E. Samuels, and V. E. Baracos Luminal Amino Acids Acutely Decrease Intestinal Mucosal Protein Synthesis and Protease mRNA in Piglets J. Nutr., October 1, 1999; 129(10): 1871 - 1878. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. M. Ray, M. J. Viar, T. B. Patel, and L. R. Johnson Interaction of asparagine and EGF in the regulation of ornithine decarboxylase in IEC-6 cells Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G773 - G780. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
