| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Gastrointestinal and Liver Physiology, Vol 270, Issue 4 634-G645, Copyright © 1996 by American Physiological Society
ARTICLES |
D. J. Philpott, D. M. McKay, P. M. Sherman and M. H. Perdue
Division of Gastroenterology, Hospital for Sick Children, Toronto, Ontario, Canada.
The effect of enteropathogenic Escherichia coli (EPEC) infection on electrophysiology of T84 cell monolayers was examined. After 18 h of infection with EPEC (E2348), transepithelial electrical resistance was decreased (30 +/- 5% of uninfected values) compared with monolayers infected with a nonpathogenic E. coli strain (104 +/- 13%). Resistance of monolayers infected with EPEC mutant strain CVD206, deficient in attaching and effacing lesion formation, was partially reduced (66 +/- 10%). In addition, permeability of EPEC-infected T84 monolayers increased compared with uninfected cells. Associated with these changes was an altered distribution of the tight junction protein, ZO-1. Taken together, these findings suggest that the barrier defect induced by EPEC was at the level of the tight junction. Adenosine 3'5'-cyclic monophosphate-stimulated chloride secretion was also diminished in EPEC-infected cells, whereas Ca2+ -dependent chloride secretion was not different from uninfected cells. These findings indicate that EPEC infection alters intestinal epithelial barrier and transport functions. Furthermore, these results provide a possible mechanism for EPEC-induced diarrheal disease.
This article has been cited by other articles:
|
|
 |
|
  K. Lewis, J. Caldwell, V. Phan, D. Prescott, A. Nazli, A. Wang, J. D. Soderholm, M. H. Perdue, P. M. Sherman, and D. M. McKay Decreased epithelial barrier function evoked by exposure to metabolic stress and nonpathogenic E. coli is enhanced by TNF-{alpha} Am J Physiol Gastrointest Liver Physiol, March 1, 2008; 294(3): G669 - G678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Boivin, D. Ye, J. C. Kennedy, R. Al-Sadi, C. Shepela, and T. Y. Ma Mechanism of glucocorticoid regulation of the intestinal tight junction barrier Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G590 - G598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nazli, A. Wang, O. Steen, D. Prescott, J. Lu, M. H. Perdue, J. D. Soderholm, P. M. Sherman, and D. M. McKay Enterocyte Cytoskeleton Changes Are Crucial for Enhanced Translocation of Nonpathogenic Escherichia coli across Metabolically Stressed Gut Epithelia Infect. Immun., January 1, 2006; 74(1): 192 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Watson, S. Ansari, H. Cameron, A. Wang, M. Akhtar, and D. M. McKay Green tea polyphenol (-)-epigallocatechin gallate blocks epithelial barrier dysfunction provoked by IFN-{gamma} but not by IL-4 Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G954 - G961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Gookin, L. L. Duckett, M. U. Armstrong, S. H. Stauffer, C. P. Finnegan, M. P. Murtaugh, and R. A. Argenzio Nitric oxide synthase stimulates prostaglandin synthesis and barrier function in C. parvum-infected porcine ileum Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G571 - G581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Berkes, V K Viswanathan, S D Savkovic, and G Hecht Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation Gut, March 1, 2003; 52(3): 439 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Tafazoli, K.-E. Magnusson, and L. Zheng Disruption of Epithelial Barrier Integrity by Salmonella enterica Serovar Typhimurium Requires Geranylgeranylated Proteins Infect. Immun., February 1, 2003; 71(2): 872 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Crane, R. A. Olson, H. M. Jones, and M. E. Duffey Release of ATP during host cell killing by enteropathogenic E. coli and its role as a secretory mediator Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G74 - G86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Johnson-Henry, J. L. Wallace, N. S. Basappa, R. Soni, G. K. P. Wu, and P. M. Sherman Inhibition of Attaching and Effacing Lesion Formation following Enteropathogenic Escherichia coli and Shiga Toxin-Producing E. coli Infection Infect. Immun., November 1, 2001; 69(11): 7152 - 7158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Simonovic, M. Arpin, A. Koutsouris, H. J. Falk-Krzesinski, and G. Hecht Enteropathogenic Escherichia coli Activates Ezrin, Which Participates in Disruption of Tight Junction Barrier Function Infect. Immun., September 1, 2001; 69(9): 5679 - 5688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zareie, P. K. Singh, E. J. Irvine, P. M. Sherman, D. M. McKay, and M. H. Perdue Monocyte/Macrophage Activation by Normal Bacteria and Bacterial Products : Implications for Altered Epithelial Function in Crohn's Disease Am. J. Pathol., March 1, 2001; 158(3): 1101 - 1109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Tafazoli, C. Q. Zeng, M. K. Estes, K.-E. Magnusson, and L. Svensson NSP4 Enterotoxin of Rotavirus Induces Paracellular Leakage in Polarized Epithelial Cells J. Virol., February 1, 2001; 75(3): 1540 - 1546. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Jepson, H. B. Schlecht, and C. B. Collares-Buzato Localization of Dysfunctional Tight Junctions in Salmonella enterica Serovar Typhimurium-Infected Epithelial Layers Infect. Immun., December 1, 2000; 68(12): 7202 - 7208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Vallance and B. B. Finlay Exploitation of host cells by enteropathogenic Escherichiacoli PNAS, August 1, 2000; 97(16): 8799 - 8806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M A Benjamin, D M McKay, P-C Yang, H Cameron, and M H Perdue Glucagon-like peptide-2 enhances intestinal epithelial barrier function of both transcellular and paracellular pathways in the mouse Gut, July 1, 2000; 47(1): 112 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Peiffer, A.-B. Blanc-Potard, M.-F. Bernet-Camard, J. Guignot, A. Barbat, and A. L. Servin Afa/Dr Diffusely Adhering Escherichia coli C1845 Infection Promotes Selective Injuries in the Junctional Domain of Polarized Human Intestinal Caco-2/TC7 Cells Infect. Immun., June 1, 2000; 68(6): 3431 - 3442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Li, E. Elliott, J. Payne, J. Isaacs, P. Gunning, and E. V. O'loughlin Shiga Toxin-Producing Escherichia coli Can Impair T84 Cell Structure and Function without Inducing Attaching/Effacing Lesions Infect. Immun., November 1, 1999; 67(11): 5938 - 5945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hecht and A. Koutsouris Enteropathogenic E. coli attenuates secretagogue-induced net intestinal ion transport but not Cl- secretion Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G781 - G788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matysiak-Budnik, K. Terpend, S. Alain, M.-J. Sanson le Pors, J.-F. Desjeux, F. Megraud, and M. Heyman Helicobacter pylori Alters Exogenous Antigen Absorption and Processing in a Digestive Tract Epithelial Cell Line Model Infect. Immun., December 1, 1998; 66(12): 5785 - 5791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. K. Collington, I. W. Booth, M. S. Donnenberg, J. B. Kaper, and S. Knutton Enteropathogenic Escherichia coli Virulence Genes Encoding Secreted Signalling Proteins Are Essential for Modulation of Caco-2 Cell Electrolyte Transport Infect. Immun., December 1, 1998; 66(12): 6049 - 6053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lu, D. J. Philpott, P. R. Saunders, M. H. Perdue, P.-C. Yang, and D. M. McKay Epithelial Ion Transport and Barrier Abnormalities Evoked by Superantigen-Activated Immune Cells Are Inhibited by Interleukin-10 but Not Interleukin-4 J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 128 - 136. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Zareie, D. M. McKay, G. G. Kovarik, and M. H. Perdue Monocyte/macrophages evoke epithelial dysfunction: indirect role of tumor necrosis factor-alpha Am J Physiol Cell Physiol, October 1, 1998; 275(4): C932 - C939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bain, R. Keller, G. K. Collington, L. R. Trabulsi, and S. Knutton Increased Levels of Intracellular Calcium Are Not Required for the Formation of Attaching and Effacing Lesions by Enteropathogenic and Enterohemorrhagic Escherichia coli Infect. Immun., August 1, 1998; 66(8): 3900 - 3908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Philpott, D. M. McKay, W. Mak, M. H. Perdue, and P. M. Sherman Signal Transduction Pathways Involved in Enterohemorrhagic Escherichia coli-Induced Alterations in T84 Epithelial Permeability Infect. Immun., April 1, 1998; 66(4): 1680 - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Nataro and J. B. Kaper Diarrheagenic Escherichia coli Clin. Microbiol. Rev., January 1, 1998; 11(1): 142 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Philpott, C. A. Ackerley, A. J. Kiliaan, M. A. Karmali, M. H. Perdue, and P. M. Sherman Translocation of verotoxin-1 across T84 monolayers: mechanism of bacterial toxin penetration of epithelium Am J Physiol Gastrointest Liver Physiol, December 1, 1997; 273(6): G1349 - G1358. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
