AJP - Gastrointestinal and Liver Physiology, Vol 271, Issue 3 454-G460, Copyright © 1996 by American Physiological Society

ARTICLES

Decreased ileal muscle contractility and increased NOS II expression induced by lipopolysaccharide

N. W. Weisbrodt, T. A. Pressley, Y. F. Li, M. J. Zembowicz, S. C. Higham, A. Zembowicz, R. F. Lodato and F. G. Moody
Department of Integrative Biology, University of Texas Medical School, Houston 77030, USA. weisbrod@girch1.med.uth.tmc.edu

This study was designed to determine if an increase in nitric oxide synthase (NOS) activity induced by lipopolysaccharide (LPS) is associated with increases in NOS II protein and mRNA abundance and with altered ileal longitudinal muscle contractility. Strips of muscle taken from LPS-treated, but not control, animals exhibited reduced in vitro contractility when L-arginine was a component of the physiological salt solution. This reduction was reversed by N omega-nitro-L-arginine (L-NNA), a competitive inhibitor of NOS. Full-thickness segments of jejunum, ileum, and colon taken 5 h after LPS injection exhibited increased NOS activity, NOS II immunoreactivity, and NOS II mRNA abundance. Increased NOS II immunoreactivity and mRNA abundance also were detected in ileal muscle strips taken from LPS-treated animals. These data confirm the reported effects of LPS on intestinal NOS activity and indicate that it can be attributed, at least in part, to an increase in NOS II mRNA and protein abundance. Furthermore, the data suggest that an LPS-induced increase in NOS II may lead to a decrease in ileal muscle contractility.

This article has been cited by other articles:

				M. C. Baccari, S. Nistri, M. G. Vannucchi, F. Calamai, and D. Bani Reversal by relaxin of altered ileal spontaneous contractions in dystrophic (mdx) mice through a nitric oxide-mediated mechanism Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R662 - R668. [Abstract] [Full Text] [PDF]

				H. T. Gan and J. D. Z. Chen Roles of nitric oxide and prostaglandins in pathogenesis of delayed colonic transit after burn injury in rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1316 - R1324. [Abstract] [Full Text] [PDF]

				M. Overhaus, S. Togel, M. A. Pezzone, and A. J. Bauer Mechanisms of polymicrobial sepsis-induced ileus Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G685 - G694. [Abstract] [Full Text] [PDF]

				K. Bian, Y. Harari, M. Zhong, M. Lai, G. Castro, N. Weisbrodt, and F. Murad Down-Regulation of Inducible Nitric-Oxide Synthase (NOS-2) During Parasite-Induced Gut Inflammation: A Path to Identify a Selective NOS-2 Inhibitor Mol. Pharmacol., April 1, 2001; 59(4): 939 - 939. [Abstract] [Full Text]

				X.-W. QU, H. WANG, I. G. DE PLAEN, R. A. ROZENFELD, and W. HSUEH Neuronal nitric oxide synthase (NOS) regulates the expression of inducible NOS in rat small intestine via modulation of nuclear factor kappa B FASEB J, February 1, 2001; 15(2): 439 - 446. [Abstract] [Full Text] [PDF]

				A Sola, J Rosello-Catafau, E Gelpi, and G Hotter Fructose-1,6-biphosphate in rat intestinal preconditioning: involvement of nitric oxide Gut, February 1, 2001; 48(2): 168 - 175. [Abstract] [Full Text] [PDF]

				Y.-P. Fan, S. Chakder, F. Gao, and S. Rattan Inducible and neuronal nitric oxide synthase involvement in lipopolysaccharide-induced sphincteric dysfunction Am J Physiol Gastrointest Liver Physiol, January 1, 2001; 280(1): G32 - G42. [Abstract] [Full Text] [PDF]

				A. M. Kahn, J. C. Allen, C. L. Seidel, D. S. Lichtenberg, T. Song, and S. Zhang Insulin increases NO-stimulated guanylate cyclase activity in cultured VSMC while raising redox potential Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E627 - E633. [Abstract] [Full Text] [PDF]

				A. M. Kahn, J. C. Allen, C. L. Seidel, and S. Zhang Insulin Inhibits Migration of Vascular Smooth Muscle Cells With Inducible Nitric Oxide Synthase Hypertension, January 1, 2000; 35(1): 303 - 306. [Abstract] [Full Text] [PDF]

				M. K. Eskandari, J. C. Kalff, T. R. Billiar, K. K. W. Lee, and A. J. Bauer LPS-induced muscularis macrophage nitric oxide suppresses rat jejunal circular muscle activity Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G478 - G486. [Abstract] [Full Text] [PDF]

				R. F. Lodato, A. R. Khan, M. J. Zembowicz, N. W. Weisbrodt, T. A. Pressley, Y.-F. Li, J. A. Lodato, A. Zembowicz, and F. G. Moody Roles of IL-1 and TNF in the decreased ileal muscle contractility induced by lipopolysaccharide Am J Physiol Gastrointest Liver Physiol, June 1, 1999; 276(6): G1356 - G1362. [Abstract] [Full Text] [PDF]

				R. R. Neviere, M. L. Pitt-Hyde, R. D. Piper, W. J. Sibbald, and R. F. Potter Microvascular perfusion deficits are not a prerequisite for mucosal injury in septic rats Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G933 - G940. [Abstract] [Full Text] [PDF]

				D. W. Mercer, A. A. Castaneda, J. W. Denning, L. Chang, and D. H. Russell Effects of endotoxin on gastric injury from luminal irritants in rats: potential roles of nitric oxide Am J Physiol Gastrointest Liver Physiol, September 1, 1998; 275(3): G449 - G459. [Abstract] [Full Text] [PDF]

				A. M. Kahn, A. Husid, T. Odebunmi, J. C. Allen, C. L. Seidel, and T. Song Insulin inhibits vascular smooth muscle contraction at a site distal to intracellular Ca2+ concentration Am J Physiol Endocrinol Metab, May 1, 1998; 274(5): E885 - E892. [Abstract] [Full Text] [PDF]

				L. E. Olsson, M. A. Wheeler, W. C. Sessa, and R. M. Weiss Bladder Instillation and Intraperitoneal Injection of Escherichia coli Lipopolysaccharide Up-regulate Cytokines and iNOS in Rat Urinary Bladder J. Pharmacol. Exp. Ther., March 1, 1998; 284(3): 1203 - 1208. [Abstract] [Full Text]

				J. F. Kuemmerle Synergistic regulation of NOS II expression by IL-1beta and TNF-alpha in cultured rat colonic smooth muscle cells Am J Physiol Gastrointest Liver Physiol, January 1, 1998; 274(1): G178 - G185. [Abstract] [Full Text] [PDF]

				A. Turler, N. T. Schwarz, E. Turler, J. C. Kalff, and A. J. Bauer MCP-1 causes leukocyte recruitment and subsequently endotoxemic ileus in rat Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G145 - G155. [Abstract] [Full Text] [PDF]