| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Gastrointestinal and Liver Physiology, Vol 271, Issue 3 531-G538, Copyright © 1996 by American Physiological Society
ARTICLES |
H. Ohnishi, S. A. Ernst, N. Wys, M. McNiven and J. A. Williams
Department of Physiology, University of Michigan, Ann Arbor 48109-0622, USA.
Rab3 proteins are members of the family of Ras-like monomeric GTP-binding proteins that have been implicated in secretion in neuronal cells. Although an isoform of Rab3 has been assumed to exist in pancreatic acini, its identity has not yet been established. We now report that Rab3D is present in rat pancreatic acini and is localized to the zymogen granule membrane. Reverse transcription-polymerase chain reaction (PCR) was used with primers based on mouse Rab3D to amplify Rab3D from rat pancreas. The PCR product without primer sites consisted of 580 base pairs and was 94% identical to the mouse Rab3D cDNA sequence previously cloned from adipocytes. Western blotting with a polyclonal antiserum raised against Rab3D-specific carboxyterminal amino acids identified Rab3D in rat pancreatic acini and revealed its concentration on zymogen granule membranes. Immunocytochemistry of pancreatic lobules showed that Rab3D localized to the apical region in a pattern similar to amylase. Confocal fluorescence microscopy of lobules double immunolabeled with antibodies to Rab3D and the granule membrane marker protein glycoprotein-2 (GP-2) revealed a similar localization of these proteins to zymogen granules. Immunocytochemistry also revealed the presence of Rab3D in chief and enterochromaffin-like cells in the stomach, acinar cells in lacrimal and parotid gland, and Paneth cells in the intestine. These results show that Rab3D is expressed in rat pancreatic acini and other exocrine secretory cells. Its location implies it may be involved in regulated exocytosis.
This article has been cited by other articles:
|
|
 |
|
  E. Evans, W. Zhang, G. Jerdeva, C.-Y. Chen, X. Chen, S. F. Hamm-Alvarez, and C. T. Okamoto Direct interaction between Rab3D and the polymeric immunoglobulin receptor and trafficking through regulated secretory vesicles in lacrimal gland acinar cells Am J Physiol Cell Physiol, March 1, 2008; 294(3): C662 - C674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Valentijn, L. van Weeren, A. Ultee, and A. J. Koster Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G165 - G177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Lapierre, K. M. Avant, C. M. Caldwell, A.-J. L. Ham, S. Hill, J. A. Williams, A. J. Smolka, and J. R. Goldenring Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1249 - G1262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guo, M. D. Sans, G. T. Gurda, S.-H. Lee, S. A. Ernst, and J. A. Williams Induction of early response genes in trypsin inhibitor-induced pancreatic growth Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G667 - G677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Seino and T. Shibasaki PKA-Dependent and PKA-Independent Pathways for cAMP-Regulated Exocytosis Physiol Rev, October 1, 2005; 85(4): 1303 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. V. Jerdeva, F. A. Yarber, M. D. Trousdale, C. J. Rhodes, C. T. Okamoto, D. A. Dartt, and S. F. Hamm-Alvarez Dominant-negative PKC-{epsilon} impairs apical actin remodeling in parallel with inhibition of carbachol-stimulated secretion in rabbit lacrimal acini Am J Physiol Cell Physiol, October 1, 2005; 289(4): C1052 - C1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Evans, O. W. Williams, M. J. Tuvim, R. Nigam, G. P. Mixides, M. R. Blackburn, F. J. DeMayo, A. R. Burns, C. Smith, S. D. Reynolds, et al. Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen-Challenged Mice Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 382 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Riedel, W. Antonin, R. Fernandez-Chacon, G. Alvarez de Toledo, T. Jo, M. Geppert, J. A. Valentijn, K. Valentijn, J. D. Jamieson, T. C. Sudhof, et al. Rab3D Is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules Mol. Cell. Biol., September 15, 2002; 22(18): 6487 - 6497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Antonin, M. Wagner, D. Riedel, N. Brose, and R. Jahn Loss of the Zymogen Granule Protein Syncollin Affects Pancreatic Protein Synthesis and Transport but Not Secretion Mol. Cell. Biol., March 1, 2002; 22(5): 1545 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Chung, G. Joberty, E. A. Gelino, I. G. Macara, and R. W. Holz Comparison of the Effects on Secretion in Chromaffin and PC12 Cells of Rab3 Family Members and Mutants. EVIDENCE THAT INHIBITORY EFFECTS ARE INDEPENDENT OF DIRECT INTERACTION WITH RABPHILIN3 J. Biol. Chem., June 18, 1999; 274(25): 18113 - 18120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Nozu, Y. Tsunoda, A. I. Ibitayo, K. N. Bitar, and C. Owyang Involvement of RhoA and its interaction with protein kinase C and Src in CCK-stimulated pancreatic acini Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G915 - G923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Tuvim, R. Adachi, J. F. Chocano, R. H. Moore, R. M. Lampert, E. Zera, E. Romero, B. J. Knoll, and B. F. Dickey Rab3D, a Small GTPase, Is Localized on Mast Cell Secretory Granules and Translocates to the Plasma Membrane upon Exocytosis Am. J. Respir. Cell Mol. Biol., January 1, 1999; 20(1): 79 - 89. [Abstract] [Full Text]
|
|
|
|
|
|
G. E. Groblewski, M. Yoshida, H. Yao, J. A. Williams, and S. A. Ernst Immunolocalization of CRHSP28 in exocrine digestive glands and gastrointestinal tissues of the rat Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G219 - G226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Groblewski, M. Yoshida, M. J. Bragado, S. A. Ernst, J. Leykam, and J. A. Williams Purification and Characterization of a Novel Physiological Substrate for Calcineurin in Mammalian Cells J. Biol. Chem., August 28, 1998; 273(35): 22738 - 22744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ohnishi, S. A. Ernst, D. I. Yule, C. W. Baker, and J. A. Williams Heterotrimeric G-protein Gq/11 Localized on Pancreatic Zymogen Granules Is Involved in Calcium-regulated Amylase Secretion J. Biol. Chem., June 20, 1997; 272(25): 16056 - 16061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Yule, S. A. Ernst, H. Ohnishi, and R. J.H. Wojcikiewicz Evidence That Zymogen Granules Are Not a Physiologically Relevant Calcium Pool. DEFINING THE DISTRIBUTION OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS IN PANCREATIC ACINAR CELLS J. Biol. Chem., April 4, 1997; 272(14): 9093 - 9098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. H. Thomas, W. B. Taft, K. M. Kaspar, and G. E. Groblewski CRHSP-28 Regulates Ca2+-stimulated Secretion in Permeabilized Acinar Cells J. Biol. Chem., July 27, 2001; 276(31): 28866 - 28872. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
