AJP - GI AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 272: G563-G574, 1997;
0193-1857/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by vom Dahl, S.
Right arrow Articles by Haussinger, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by vom Dahl, S.
Right arrow Articles by Haussinger, D.

AJP - Gastrointestinal and Liver Physiology, Vol 272, Issue 3 563-G574, Copyright © 1997 by American Physiological Society

ARTICLES

Evidence for a phloretin-sensitive glycerol transport mechanism in the perfused rat liver

S. vom Dahl and D. Haussinger
Department of Gastroenterology, Hepatology, and Infectious Diseases, Universitat Dusseldorf, Germany.

Glycerol, water, and urea transport were studied in the perfused rat liver. When hypertonic glycerol (100 mM) was infused into the perfused rat liver, rapid equilibration of glycerol between the extracellular and intracellular space occurred and only small effects of osmotic water fluxes on liver mass and extracellular ion concentrations were observed. However, when phloretin (0.2 mmol/l) was present, addition (or removal) of glycerol (100 mmol/l) induced within seconds a marked and transient decrease (or increase) of both liver mass and effluent Na+ and K+ concentrations, suggestive of transient osmotic water shifts out of and/or into the cells. These effects were observed with glycerol but not with infusion of 1,2-propanediol, 1,3-propanediol, or ethanediol (100 mmol/l). Calculation of permeability coefficients for hepatocytes in the perfused rat liver revealed that in the absence of a transmembrane glycerol concentration gradient the glycerol permeability was decreased from 0.146 +/- 0.005 (n = 14) to 0.055 +/- 0.016 microm/s (n = 4) in the presence of phloretin (200 microM). Water permeability was not affected by phloretin [0.146 +/- 0.005 under control conditions (n = 12) vs. 0.157 +/- 0.006 microm/s (n = 4) in the presence of phloretin]. Urea permeability was 0.131 +/- 0.008 microm/s (n = 5) and decreased to 0.061 +/- 0.003 microm/s in the presence ofphloretin. Glycerol and water permeability were inhibited in the presence of hypertonic glucose (200 mM) by 47 and 27%, respectively, but urea permeability was not affected by glucose.


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
J. M. Sands
Molecular Approaches to Urea Transporters
J. Am. Soc. Nephrol., November 1, 2002; 13(11): 2795 - 2806.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. D. Klein, P. Rouillard, B. R. Roberts, and J. M. Sands
Acidosis Mediates the Upregulation of UT-A Protein in Livers from Uremic Rats
J. Am. Soc. Nephrol., March 1, 2002; 13(3): 581 - 587.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. D. KLEIN, R. T. TIMMER, P. ROUILLARD, J. L. BAILEY, and J. M. SANDS
UT-A Urea Transporter Protein Expressed in Liver: Upregulation byUremia
J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2076 - 2083.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Roudier, J.-M. Verbavatz, C. Maurel, P. Ripoche, and F. Tacnet
Evidence for the Presence of Aquaporin-3 in Human Red Blood Cells
J. Biol. Chem., April 3, 1998; 273(14): 8407 - 8412.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online