AJP - Gastrointestinal and Liver Physiology, Vol 272, Issue 6 1540-G1549, Copyright © 1997 by American Physiological Society

ARTICLES

Temporal expression of trefoil peptides in the TGF-alpha knockout mouse after gastric ulceration

G. A. Cook, N. D. Yeomans and A. S. Giraud
Department of Medicine, University of Melbourne at Western Hospital, Footscray, Australia.

Trefoil peptides are gut peptides that have been implicated in the repair of the gastric mucosa after injury. Previous studies suggest that epidermal growth factor (EGF) receptor ligands may induce the expression of trefoil peptides. Because transforming growth factor-alpha (TGF-alpha) is a major EGF receptor ligand in the gut, we tested the hypothesis that mice with a TGF-alpha null mutation (knockout) would have reduced trefoil peptide expression compared with wild-type controls after gastric ulceration. The rate of macroscopic ulcer healing was the same in knockout and wild-type mice. Spasmolytic polypeptide (SP) and intestinal trefoil factor (ITF) expression were quantified in tissue and gastric lavage. SP and ITF levels in tissue fell within 48 h of ulceration (P < 0.05), but secretion into gastric juice was unchanged. ITF peptide expression was increased (as was SP expression) in wild-type but not knockout mice 42 and 72 days after injury (P < 0.05). The induction of SP and ITF expression in the latter stages of injury repair has a TGF-alpha-dependent component and suggests a role for these peptides in gastric differentiation and cell positioning.

This article has been cited by other articles:

				A. Andoh, K. Kinoshita, I. Rosenberg, and D. K. Podolsky Intestinal Trefoil Factor Induces Decay-Accelerating Factor Expression and Enhances the Protective Activities Against Complement Activation in Intestinal Epithelial Cells J. Immunol., October 1, 2001; 167(7): 3887 - 3893. [Abstract] [Full Text] [PDF]

				T. Tanaka, H. Kohno, S.-i. Yoshitani, S. Takashima, A. Okumura, A. Murakami, and M. Hosokawa Ligands for Peroxisome Proliferator-activated Receptors {{alpha}} and {{gamma}} Inhibit Chemically Induced Colitis and Formation of Aberrant Crypt Foci in Rats1 Cancer Res., March 1, 2001; 61(6): 2424 - 2428. [Abstract] [Full Text]

				A. S. Giraud Lessons From Genetically Engineered Animal Models: X. Trefoil peptide and EGF receptor/ligand transgenic mice Am J Physiol Gastrointest Liver Physiol, April 1, 2000; 278(4): G501 - G506. [Abstract] [Full Text] [PDF]

				W-M WONG, R J PLAYFORD, and N A WRIGHT Peptide gene expression in gastrointestinal mucosal ulceration: ordered sequence or redundancy? Gut, February 1, 2000; 46(2): 286 - 292. [Full Text] [PDF]

				C. J. Xian, G. S. Howarth, C. E. Mardell, J. C. Cool, M. Familari, L. C. Read, and A. S. Giraud Temporal changes in TFF3 expression and jejunal morphology during methotrexate-induced damage and repair Am J Physiol Gastrointest Liver Physiol, October 1, 1999; 277(4): G785 - G795. [Abstract] [Full Text] [PDF]

				M P A Ebert, J Hoffmann, C Haeckel, K Rutkowski, R M Schmid, M Wagner, G Adler, H U Schulz, A Roessner, W Hoffmann, et al. Induction of TFF1 gene expression in pancreas overexpressing transforming growth factor alpha Gut, July 1, 1999; 45(1): 105 - 111. [Abstract] [Full Text] [PDF]

				C P Tran, G A Cook, N D Yeomans, L Thim, and A S Giraud Trefoil peptide TFF2 (spasmolytic polypeptide) potently accelerates healing and reduces inflammation in a rat model of colitis Gut, May 1, 1999; 44(5): 636 - 642. [Abstract] [Full Text] [PDF]