AJP - GI AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 273: G139-G146, 1997;
0193-1857/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Castell, J. V.
Right arrow Articles by Poppe, G. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castell, J. V.
Right arrow Articles by Poppe, G. E.

AJP - Gastrointestinal and Liver Physiology, Vol 273, Issue 1 139-G146, Copyright © 1997 by American Physiological Society

ARTICLES

Intestinal absorption of undegraded proteins in men: presence of bromelain in plasma after oral intake

J. V. Castell, G. Friedrich, C. S. Kuhn and G. E. Poppe
Unidad de Hepatologia Experimental, Hospital Universitario La Fe, Valencia, Spain.

The human adult intestinal epithelium has traditionally been described as nonpermeable to proteins. However, indirect evidence suggests that reduced absorption of undegraded proteins might take place under physiological conditions. Using bromelain (an enzyme obtained from pineapple stems) as a model protein, we studied the extent of this mucosal permeation in 19 healthy men. The protein was detected in plasma by immunoassay and by its proteolytic activity after oral administration. The estimated plasma half-life was 6-9 h. After oral multidosing (3 g/day), plasma concentration reached as much as 5,000 pg/ml by 48 h. From the plasma concentration curve, it could be estimated that an average of 10.8 micrograms of bromelain was present in plasma in the 3- to 51-h period. The presence of undegraded bromelain in plasma was shown unequivocally by immunoprecipitation of plasma samples with antibromelain antibodies, followed by gel electrophoresis and immunodetection. Moreover, the enzyme retained its biological activity, at least in part. Circulating bromelain was found associated with alpha 2-macroglobulin and alpha 1-antichymotrypain. The results of this work confirm the existence of a small but significant intestinal transport of undegraded proteins in healthy men.


This article has been cited by other articles:


Home page
 J. Am. Coll. Nutr.Home page
A. Iaconelli, L. Fiorentini, S. Bruschi, F. Rossi, G. Mingrone, and G. Piva
Absence of Allergic Reactions to Egg White Lysozyme Additive in Grana Padano Cheese
J. Am. Coll. Nutr., April 1, 2008; 27(2): 326 - 331.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Nishikawa, S. Hasegawa, F. Yamashita, Y. Takakura, and M. Hashida
Electrical charge on protein regulates its absorption from the rat small intestine
Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G711 - G719.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. Oesser, M. Adam, W. Babel, and J. Seifert
Oral Administration of 14C Labeled Gelatin Hydrolysate Leads to an Accumulation of Radioactivity in Cartilage of Mice (C57/BL)
J. Nutr., October 1, 1999; 129(10): 1891 - 1895.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online