| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Gastrointestinal and Liver Physiology, Vol 273, Issue 3 679-G685, Copyright © 1997 by American Physiological Society
ARTICLES |
Y. Li, Y. Hao and C. Owyang
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109, USA.
Cholecystokinin (CCK) receptors are found on vagal afferent fibers. In pancreatic acini, CCK receptors exist in high- and low-affinity states. The aim of this study was to identify the vagal CCK-A receptor affinity state that mediates the effect of CCK on pancreatic protein secretion. Using a rat model with a pancreatic-biliary cannula, we studied the effects of CCK-JMV-180 on exocrine pancreatic function. CCK-JMV-180 acts as an agonist on high-affinity CCK receptors and as an antagonist on low-affinity CCK receptors. Infusion of CCK-JMV-180 (22-88 micrograms.kg-1.h-1) caused dose-dependent increases in pancreatic protein secretion, which were blocked by the CCK-A receptor antagonist L-364,718. Acute vagotomy in anesthetized rats and perivagal application of capsaicin in conscious rats abolished pancreatic responses to CCK-JMV-180 at 22 and 44 micrograms.kg-1.h-1. CCK-JMV-180 did not reduce pancreatic responses to CCK octapeptide infusion at 20 and 40 pmol.kg-1.h-1. To demonstrate that endogenously released CCK also acts on high-affinity CCK-A receptors, we showed that in conscious rats intraduodenal infusion of 18% casein produced a threefold increase in protein secretion and elevated plasma CCK levels from 0.7 to 8.4 pM. Infusion of CCK-JMV-180 at 44 micrograms.kg-1.h-1 failed to inhibit pancreatic responses to casein. In separate studies, perivagal application of 1% capsaicin inhibited 95% and 90% of the pancreatic responses to casein and casein combined with intravenous CCK-JMV-180, respectively. The neurotoxic effect of capsaicin on small-diameter sensory vagal fibers was verified by immunohistochemical and retrograde tracing studies. In conclusion, we demonstrated that in contrast to their effect on satiety, which is mediated by vagal low-affinity CCK-A receptors, exogenous CCK and endogenous CCK under physiological conditions act through high-affinity CCK-A receptors to mediate pancreatic protein secretion. These findings suggest that different affinity states of the vagal CCK receptors mediate different digestive functions.
This article has been cited by other articles:
|
|
 |
|
  A. T. Stearns, A. Balakrishnan, J. Rounds, D. B. Rhoads, S. W. Ashley, and A. Tavakkolizadeh Capsaicin-sensitive vagal afferents modulate posttranscriptional regulation of the rat Na+/glucose cotransporter SGLT1 Am J Physiol Gastrointest Liver Physiol, April 1, 2008; 294(4): G1078 - G1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wan, F. H. Coleman, and R. A. Travagli Cholecystokinin-8s excites identified rat pancreatic-projecting vagal motoneurons Am J Physiol Gastrointest Liver Physiol, August 1, 2007; 293(2): G484 - G492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guo, M. D. Sans, G. T. Gurda, S.-H. Lee, S. A. Ernst, and J. A. Williams Induction of early response genes in trypsin inhibitor-induced pancreatic growth Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G667 - G677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Wang and Z. J. Cui How does cholecystokinin stimulate exocrine pancreatic secretion? From birds, rodents, to humans Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R666 - R678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dufresne, C. Seva, and D. Fourmy Cholecystokinin and gastrin receptors. Physiol Rev, July 1, 2006; 86(3): 805 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Browning, F. H. Coleman, and R. A. Travagli Effects of pancreatic polypeptide on pancreas-projecting rat dorsal motor nucleus of the vagus neurons Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G209 - G219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Browning, F. H. Coleman, and R. A. Travagli Characterization of pancreas-projecting rat dorsal motor nucleus of vagus neurons Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G950 - G955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Simasko and R. C. Ritter Cholecystokinin activates both A- and C-type vagal afferent neurons Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1204 - G1213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nishi, H. Hara, and F. Tomita Soybean {beta}-Conglycinin Peptone Suppresses Food Intake and Gastric Emptying by Increasing Plasma Cholecystokinin Levels in Rats J. Nutr., February 1, 2003; 133(2): 352 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Simasko, J. Wiens, A. Karpiel, M. Covasa, and R. C. Ritter Cholecystokinin increases cytosolic calcium in a subpopulation of cultured vagal afferent neurons Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2002; 283(6): R1303 - R1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. O. Lankisch, Y. Tsunoda, Y. Lu, and C. Owyang Characterization of CCKA receptor affinity states and Ca2+ signal transduction in vagal nodose ganglia Am J Physiol Gastrointest Liver Physiol, June 1, 2002; 282(6): G1002 - G1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Sartor and A. J. M. Verberne Cholecystokinin selectively affects presympathetic vasomotor neurons and sympathetic vasomotor outflow Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1174 - R1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, X. Y. Wu, J. X. Zhu, and C. Owyang Intestinal serotonin acts as paracrine substance to mediate pancreatic secretion stimulated by luminal factors Am J Physiol Gastrointest Liver Physiol, October 1, 2001; 281(4): G916 - G923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Deng, D. R. Guarita, M. R. A. Pedroso, C. Kreiss, P. G. Wood, A. F. Sved, and D. C. Whitcomb PYY inhibits CCK-stimulated pancreatic secretion through the area postrema in unanesthetized rats Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R645 - R653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, H. Zheng, and H.-R. Berthoud Functional vagal input to chemically identified neurons in pancreatic ganglia as revealed by Fos expression Am J Physiol Endocrinol Metab, November 1, 1999; 277(5): E958 - E964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, J. Zhu, and C. Owyang Electrical physiological evidence for highand low-affinity vagal CCK-A receptors Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G469 - G477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Holzer II. The elusive action of capsaicin on the vagus nerve Am J Physiol Gastrointest Liver Physiol, July 1, 1998; 275(1): G8 - G13. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
