AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 273: G812-G823, 1997;
0193-1857/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rao, R. K.
Right arrow Articles by Holycross, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rao, R. K.
Right arrow Articles by Holycross, M.
Vol. 273, Issue 4, G812-G823, October 1997

Oxidant-induced disruption of intestinal epithelial barrier function: role of protein tyrosine phosphorylation

R. K. Rao, R. D. Baker, S. S. Baker, A. Gupta, and M. Holycross

Division of Gastroenterology, Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina 29425

The effect of hydrogen peroxide (H2O2) on intestinal epithelial barrier function was examined in Caco-2 and T84 cell monolayers. H2O2 reduced transepithelial electrical resistance (TER) of Caco-2 and T84 cell monolayers. This decrease in TER was associated with a decrease in dilution potential and an increase in [3H]mannitol permeability, suggesting an H2O2-induced disruption of the paracellular junctional complexes. H2O2 administration also induced tyrosine phosphorylation of several proteins (at the molecular mass ranges of 50-90, 100-130, and 150-180 kDa) in Caco-2 cell monolayers. Phenylarsine oxide and sodium orthovanadate, inhibitors of protein tyrosine phosphatase, decreased TER and increased mannitol permeability and protein tyrosine phosphorylation (PTP). A low concentration of sodium orthovanadate also potentiated the effect of H2O2 on TER, dilution potential, mannitol permeability, and PTP. Pretreatment with genistein (30-300 µM) and tyrphostin (100 µM) inhibited the effect of H2O2 on TER, dilution potential, mannitol permeability, and PTP. These studies show that H2O2 increases the epithelial permeability by disrupting paracellular junctional complexes, most likely by a PTP-dependent mechanism.

Caco-2 cell; hydrogen peroxide; tight junction; protein tyrosine phosphatase; tyrosine kinase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Seth, F. Yan, D. B. Polk, and R. K. Rao
Probiotics ameliorate the hydrogen peroxide-induced epithelial barrier disruption by a PKC- and MAP kinase-dependent mechanism
Am J Physiol Gastrointest Liver Physiol, April 1, 2008; 294(4): G1060 - G1069.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Levy, K. Trudel, M. Bendayan, E. Seidman, E. Delvin, M. Elchebly, J.-C. Lavoie, L.-P. Precourt, D. Amre, and D. Sinnett
Biological role, protein expression, subcellular localization, and oxidative stress response of paraoxonase 2 in the intestine of humans and rats
Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1252 - G1261.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. Sheth, N. Delos Santos, A. Seth, N. F. LaRusso, and R. K. Rao
Lipopolysaccharide disrupts tight junctions in cholangiocyte monolayers by a c-Src-, TLR4-, and LBP-dependent mechanism
Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G308 - G318.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. W. Musch, M. M. Walsh-Reitz, and E. B. Chang
Roles of ZO-1, occludin, and actin in oxidant-induced barrier disruption
Am J Physiol Gastrointest Liver Physiol, February 1, 2006; 290(2): G222 - G231.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
Z. Liu and B. Chen
Copper treatment alters the barrier functions of human intestinal Caco-2 cells: involving tight junctions and P-glycoprotein
Human and Experimental Toxicology, August 1, 2004; 23(8): 369 - 377.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
S. Basuroy, P. Sheth, D. Kuppuswamy, S. Balasubramanian, R. M. Ray, and R. K. Rao
Expression of Kinase-inactive c-Src Delays Oxidative Stress-induced Disassembly and Accelerates Calcium-mediated Reassembly of Tight Junctions in the Caco-2 Cell Monolayer
J. Biol. Chem., March 28, 2003; 278(14): 11916 - 11924.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
F. Courtois, E. G Seidman, E. Delvin, C. Asselin, S. Bernotti, M. Ledoux, and E. Levy
Membrane peroxidation by lipopolysaccharide and iron-ascorbate adversely affects Caco-2 cell function: beneficial role of butyric acid
Am. J. Clinical Nutrition, March 1, 2003; 77(3): 744 - 750.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. Schmelz, V. J. Schmid, and A. R. Parrish
Selective Disruption of Cadherin/Catenin Complexes by Oxidative Stress in Precision-Cut Mouse Liver Slices
Toxicol. Sci., June 1, 2001; 61(2): 389 - 394.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. J. Atkinson and R. K. Rao
Role of protein tyrosine phosphorylation in acetaldehyde-induced disruption of epithelial tight junctions
Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1280 - G1288.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Banan, J. Z. Fields, Y. Zhang, and A. Keshavarzian
Key role of PKC and Ca2+ in EGF protection of microtubules and intestinal barrier against oxidants
Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G828 - G843.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Karczewski and J. Groot
Molecular Physiology and Pathophysiology of Tight Junctions III. Tight junction regulation by intracellular messengers: differences in response within and between epithelia
Am J Physiol Gastrointest Liver Physiol, October 1, 2000; 279(4): G660 - G665.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. K. Rao, L. Li, R. D. Baker, S. S. Baker, and A. Gupta
Glutathione oxidation and PTPase inhibition by hydrogen peroxide in Caco-2 cell monolayer
Am J Physiol Gastrointest Liver Physiol, August 1, 2000; 279(2): G332 - G340.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
I. Peiffer, A.-B. Blanc-Potard, M.-F. Bernet-Camard, J. Guignot, A. Barbat, and A. L. Servin
Afa/Dr Diffusely Adhering Escherichia coli C1845 Infection Promotes Selective Injuries in the Junctional Domain of Polarized Human Intestinal Caco-2/TC7 Cells
Infect. Immun., June 1, 2000; 68(6): 3431 - 3442.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
C. L. Wells, R. P. Jechorek, K. M. Kinneberg, S. M. Debol, and S. L. Erlandsen
The Isoflavone Genistein Inhibits Internalization of Enteric Bacteria by Cultured Caco-2 and HT-29 Enterocytes
J. Nutr., March 1, 1999; 129(3): 634 - 640.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
J. C. Parker, C. L. Ivey, and A. Tucker
Phosphotyrosine phosphatase and tyrosine kinase inhibition modulate airway pressure-induced lung injury
J Appl Physiol, November 1, 1998; 85(5): 1753 - 1761.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. N. Meyer, C. Schwesinger, J. Ye, B. M. Denker, and S. K. Nigam
Reassembly of the Tight Junction after Oxidative Stress Depends on Tyrosine Kinase Activity
J. Biol. Chem., June 15, 2001; 276(25): 22048 - 22055.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online